Key features and details
- Rabbit polyclonal to HMBS/PBGD
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HMBS/PBGD antibody
See all HMBS/PBGD primary antibodies
DescriptionRabbit polyclonal to HMBS/PBGD
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Cat, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat cell lysates
This product was previously labelled as HMBS
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab185929 is affinity purified using an epitope specific to HMBS/PBGD immobilized on solid support
Our Abpromise guarantee covers the use of ab185929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 39 kDa.|
FunctionTetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Tissue specificityIsoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
Involvement in diseaseDefects in HMBS are the cause of acute intermittent porphyria (AIP) [MIM:176000]. AIP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AIP is an autosomal dominant form of hepatic porphyria characterized by acute attacks of neurological dysfunctions with abdominal pain, hypertension, tachycardia, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
Sequence similaritiesBelongs to the HMBS family.
- Information by UniProt
- HEM3_HUMAN antibody
- HMBS antibody
- Hydroxymethylbilane synthase antibody
Western blot analysis of Immunoprecipitates.
Immunoprecipitation of 293T whole cell lysate (1 mg for IP; 20% of IP loaded) using ab185929 at 6 µg/mg lysate for IP and at 1 µg/ml for blotting
Detection: Chemiluminescence with an exposure time of 10 seconds.
All lanes : Anti-HMBS/PBGD antibody (ab185929) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 3 minutes
ab185929 has not yet been referenced specifically in any publications.