Anti-HMBS/PBGD antibody (ab232927)
Key features and details
- Rabbit polyclonal to HMBS/PBGD
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-HMBS/PBGD antibody
See all HMBS/PBGD primary antibodies -
Description
Rabbit polyclonal to HMBS/PBGD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human HMBS/PBGD aa 50-350. (Expressed in E.coli).
Database link: P08397 -
Positive control
- WB: Recombinant human HMBS/PBGD protein; HeLa, HEK-293T and HepG2 cell lysates; Pig heart lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 44.12% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab232927 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 5 - 20 µg/ml.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa.
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Notes |
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ICC/IF
Use a concentration of 5 - 20 µg/ml. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa. |
Target
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Function
Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps. -
Tissue specificity
Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells. -
Pathway
Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4. -
Involvement in disease
Defects in HMBS are the cause of acute intermittent porphyria (AIP) [MIM:176000]. AIP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AIP is an autosomal dominant form of hepatic porphyria characterized by acute attacks of neurological dysfunctions with abdominal pain, hypertension, tachycardia, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors. -
Sequence similarities
Belongs to the HMBS family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 515614 Cow
- Entrez Gene: 3145 Human
- Entrez Gene: 15288 Mouse
- Entrez Gene: 396581 Pig
- Entrez Gene: 25709 Rat
- Omim: 609806 Human
- SwissProt: Q2KIN5 Cow
- SwissProt: P08397 Human
see all -
Alternative names
- HEM3_HUMAN antibody
- HMBS antibody
- Hydroxymethylbilane synthase antibody
see all
Images
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Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml + Recombinant human HMBS/PBGD protein
Secondary
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa -
All lanes : Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Pig heart lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa -
Paraffin-embedded human pancreatic cancer tissue stained for HMBS/PBGD using ab232927 at 10 μg/ml in immunohistochemical analysis. DAB staining.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HMBS/PBGD (green) using ab232927 at 20 μg/ml in ICC/IF, followed by FITC staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab232927 has not yet been referenced specifically in any publications.