Key features and details
- Rabbit polyclonal to HMBS/PBGD
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-HMBS/PBGD antibody
See all HMBS/PBGD primary antibodies
DescriptionRabbit polyclonal to HMBS/PBGD
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment (His-T7-tag) corresponding to Human HMBS/PBGD aa 85-337. (Expressed in E.coli).
LEKNEVDLVVHSLKDLPTVLPPGFTIGAICKRENPHDAVVFHPKFVGKTL ETLPEKSVVGTSSLRRAAQLQRKFPHLEFRSIRGNLNTRLRKLDEQQEFS AIILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDL VGVLHDPETLLRCIAERAFLRHLEGGCSVPVAVHTAMKDGQLYLTGGVWS LDGSDSIQETMQATIHVPAQHEDGPEDDPQLVGITARNIPRGPQLAAQNL GIS
Database link: P08397
- WB: Recombinant human HMBS/PBGD protein; HeLa, HEK-293T and HepG2 cell lysates; Pig heart lysate. IHC-P: Human pancreatic cancer tissue. ICC/IF: HeLa cells.
This product was previously labelled as HMBS
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab232927 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab232927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 - 20 µg/ml.|
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 39 kDa.|
FunctionTetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
Tissue specificityIsoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.
PathwayPorphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
Involvement in diseaseDefects in HMBS are the cause of acute intermittent porphyria (AIP) [MIM:176000]. AIP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AIP is an autosomal dominant form of hepatic porphyria characterized by acute attacks of neurological dysfunctions with abdominal pain, hypertension, tachycardia, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
Sequence similaritiesBelongs to the HMBS family.
- Information by UniProt
- HEM3_HUMAN antibody
- HMBS antibody
- Hydroxymethylbilane synthase antibody
Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml + Recombinant human HMBS/PBGD protein
HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
All lanes : Anti-HMBS/PBGD antibody (ab232927) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 4 : Pig heart lysate
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Paraffin-embedded human pancreatic cancer tissue stained for HMBS/PBGD using ab232927 at 10 μg/ml in immunohistochemical analysis. DAB staining.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HMBS/PBGD (green) using ab232927 at 20 μg/ml in ICC/IF, followed by FITC staining.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232927 has not yet been referenced specifically in any publications.