• Product name

    Anti-HMBS/PBGD antibody [EPR8105]
    See all HMBS/PBGD primary antibodies
  • Description

    Rabbit monoclonal [EPR8105] to HMBS/PBGD
  • Host species

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human HMBS/PBGD aa 300-400.
    Database link: P08397

  • Positive control

    • HepG2, HeLa, U937, and K562 cell lysates
  • General notes



     This product was previously labelled as HMBS


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab129092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 42-44 kDa (predicted molecular weight: 39 kDa).Can be blocked with Recombinant Human HMBS/PBGD protein (ab123176).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Function

      Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.
    • Tissue specificity

      Isoform 1 is ubiquitously expressed. Isoform 2 is found only in erythroid cells.
    • Pathway

      Porphyrin metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.
    • Involvement in disease

      Defects in HMBS are the cause of acute intermittent porphyria (AIP) [MIM:176000]. AIP is a form of porphyria. Porphyrias are inherited defects in the biosynthesis of heme, resulting in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver. AIP is an autosomal dominant form of hepatic porphyria characterized by acute attacks of neurological dysfunctions with abdominal pain, hypertension, tachycardia, and peripheral neuropathy. Most attacks are precipitated by drugs, alcohol, caloric deprivation, infections, or endocrine factors.
    • Sequence similarities

      Belongs to the HMBS family.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • HEM3_HUMAN antibody
      • HMBS antibody
      • Hydroxymethylbilane synthase antibody
      • PBG D antibody
      • PBG-D antibody
      • PBGD antibody
      • PORC antibody
      • Porphobilinogen deaminase antibody
      • porphyria, acute; Chester type antibody
      • Pre uroporphyrinogen synthase antibody
      • Pre-uroporphyrinogen synthase antibody
      • UPS antibody
      • Uroporphyrinogen I synthase antibody
      • Uroporphyrinogen I synthetase antibody
      see all


    • All lanes : Anti-HMBS/PBGD antibody [EPR8105] (ab129092) at 1/1000 dilution

      Lane 1 : HepG2 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : U937 cell lysates
      Lane 4 : K562 cell lysates

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 39 kDa
      Observed band size: 42-44 kDa
      why is the actual band size different from the predicted?

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:

    • Chen B  et al. Identification and characterization of 40 novel hydroxymethylbilane synthase mutations that cause acute intermittent porphyria. J Inherit Metab Dis 42:186-194 (2019). Read more (PubMed: 30740734) »
    See 1 Publication for this product

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