Overview

  • Product name

    HMG-CoA Reductase Activity Assay Kit (Colorimetric)
  • Detection method

    Colorimetric
  • Sample type

    Purified protein
  • Assay type

    Enzyme activity
  • Assay time

    0h 10m
  • Product overview

    HMG-CoA Reductase Activity Assay Kit (Colorimetric) (ab204701) is suitable for measuring activity of purified HMG-CoA reductase or for screening inhibitors/activators of HMG-CoA reductase. It is based on the consumption of NADPH by the enzyme, which can be measured by the decrease of absorbance at OD=340 nm. The limit of detection is below 0.05 mU.

  • Notes

    HMG-CoA reductase (3-hydroxy-3-methyl-glutaryl-CoA reductase or HMGR) (EC 1.1.1.34) is the rate-controlling enzyme of the mevalonate pathway, the metabolic pathway that produces cholesterol from acetyl-CoA. In an NADPH-dependent reaction, HMG-CoA reductase reduces HMG-CoA to generate mevalonate and CoA. The enzyme is target of a group of cholesterol-lowering drugs known as statins. Inhibition of HMG-CoA reductase induces expression of LDL receptors in the liver, which lowers plasma concentration of cholesterol.

  • Platform

    Microplate reader

Properties

Images

  • Activity of HMG-CoA Reductase (positive control included in the kit) compared to the background control with no enzyme.

  • Comparison of HMG-COA Reductase activity in absence or presence of specific inhibitors such as atorvastatin (100 µM) and pravastatin (10 ng/µl); ND: Not Detectable.

Protocols

References

This product has been referenced in:

  • Xu C  et al. CSA13 inhibits colitis-associated intestinal fibrosis via a formyl peptide receptor like-1 mediated HMG-CoA reductase pathway. Sci Rep 7:16351 (2017). Read more (PubMed: 29180648) »
See 1 Publication for this product

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