Overview

  • Product name
  • Description
    Rabbit polyclonal to HMG1 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HMG1 alpha. The exact sequence is proprietary.
    Database link: P09429

  • Positive control
    • SKBR3, HepG2 and HeLa cell line lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab175292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/20 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/500 - 1/2000. Predicted molecular weight: 25 kDa.
IHC-P 1/50 - 1/200.

Target

  • Relevance
    High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA binding nuclear protein. Mammalian HMG1 has two homologous DNA binding domains HMG boxes, A and B (each of 80 90 aminoacid residues), linked by a short basic region to an acidic C terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release.
  • Cellular localization
    Nuclear, condensed chromosome, nucleolus, nucleoplasm.
  • Database links
  • Alternative names
    • Amphoterin antibody
    • DKFZp686A04236 antibody
    • High mobility group (nonhistone chromosomal) protein 1 antibody
    • High mobility group box 1 antibody
    • High mobility group protein 1 antibody
    • High mobility group protein B1 antibody
    • HMG 1 antibody
    • HMG1 antibody
    • HMG3 antibody
    • HMGB1 antibody
    • SBP 1 antibody
    • Sulfoglucoronyl carbohydrate binding protein antibody
    see all

Images

  • All lanes : Anti-HMG1 alpha antibody (ab175292) at 1/500 dilution

    Lane 1 : SKBR3 cell line lysate
    Lane 2 : HepG2 cell line lysate
    Lane 3 : HeLa cell line lysate

    Predicted band size: 25 kDa

References

ab175292 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab175292.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up