Product nameAnti-HMG1 alpha antibody
DescriptionRabbit polyclonal to HMG1 alpha
Tested applicationsSuitable for: Flow Cyt, WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HMG1 alpha. The exact sequence is proprietary.
Database link: P09429
- SKBR3, HepG2 and HeLa cell line lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab175292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/20 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 25 kDa.|
|IHC-P||1/50 - 1/200.|
RelevanceHigh mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA binding nuclear protein. Mammalian HMG1 has two homologous DNA binding domains HMG boxes, A and B (each of 80 90 aminoacid residues), linked by a short basic region to an acidic C terminal domain containing 30 consecutive Asp and Glu residues. HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compaction or unwinding, or by direct contacts with distinct cellular proteins. HMG1 can act as a repressor, by interacting with TBP to block pre initiation complex formation or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences. Most recently, it was discovered that HMG1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release.
Cellular localizationNuclear, condensed chromosome, nucleolus, nucleoplasm.
- Amphoterin antibody
- DKFZp686A04236 antibody
- High mobility group (nonhistone chromosomal) protein 1 antibody
ab175292 has not yet been referenced specifically in any publications.