Key features and details
- Rabbit polyclonal to HMG4
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HMG4 antibody
See all HMG4 primary antibodies
DescriptionRabbit polyclonal to HMG4
Tested applicationsSuitable for: IHC-P, WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).
- HeLa and 293T whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionBinds preferentially single-stranded DNA and unwinds double stranded DNA.
Tissue specificityExpressed predominantly in placenta.
Sequence similaritiesBelongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- chromosomal protein, Nonhistone, HMG4 antibody
- High mobility group (nonhistone chromosomal) protein 4 antibody
- High mobility group box 3 antibody
All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
ab72544 has not yet been referenced specifically in any publications.