• Product name

  • Description

    Rabbit polyclonal to HMG4
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 125 and 175 of human HMG4 (NP_005333.2).

  • Positive control

    • HeLa and 293T whole cell lysates.



Our Abpromise guarantee covers the use of ab72544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function

    Binds preferentially single-stranded DNA and unwinds double stranded DNA.
  • Tissue specificity

    Expressed predominantly in placenta.
  • Sequence similarities

    Belongs to the HMGB family.
    Contains 2 HMG box DNA-binding domains.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • chromosomal protein, Nonhistone, HMG4 antibody
    • High mobility group (nonhistone chromosomal) protein 4 antibody
    • High mobility group box 3 antibody
    • High mobility group protein 2a antibody
    • High mobility group protein 4 antibody
    • High mobility group protein B3 antibody
    • High mobility group protein HMG4 antibody
    • HMG 4 antibody
    • HMG-2a antibody
    • HMG-4 antibody
    • HMG2A antibody
    • HMGB 3 antibody
    • Hmgb3 antibody
    • HMGB3_HUMAN antibody
    • MGC90319 antibody
    • Non histone chromosomal protein antibody
    • Nonhistone chromosomal protein HMG4 antibody
    see all


  • All lanes : Anti-HMG4 antibody (ab72544) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 23 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin basal cell carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling HMG4 with ab72544 at 1/200 (1µg/ml). Detection: DAB.
  • Detection of Human HMG4 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72544 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.


ab72544 has not yet been referenced specifically in any publications.

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