Product nameAnti-HMG4 antibody [EP2839Y]
See all HMG4 primary antibodies
DescriptionRabbit monoclonal [EP2839Y] to HMG4
Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HMG4 aa 150-250 (C terminal). The exact sequence is proprietary.
- 293T, HeLa and human placenta cell lysates, human placenta tissue
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75782 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2500 - 1/5000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds preferentially single-stranded DNA and unwinds double stranded DNA.
Tissue specificityExpressed predominantly in placenta.
Sequence similaritiesBelongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- chromosomal protein, Nonhistone, HMG4 antibody
- High mobility group (nonhistone chromosomal) protein 4 antibody
- High mobility group box 3 antibody
All lanes : Anti-HMG4 antibody [EP2839Y] (ab75782) at 1/5000 dilution
Lane 1 : 293T cell
Lane 2 : HeLa cell
Lane 3 : human placenta cell
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa (possible this band results from an immunoprecipitated sample, it is the ~55kDa heavy chain of the antibody used for the IP)
Immunohistochemical analysis of paraffin-embedded human placenta using ab75782 at 1/100-1/250 dilution and with the antigen retrieval step.