Overview

  • Product name

  • Description

    Goat polyclonal to HMGA1
  • Host species

    Goat
  • Specificity

    ab223658 is expected to recognize isoform a (also called HMG-I; NP_665906.1; NP_665908.1) and isoform b (also called HMG-Y; NP_002122.1; NP_665909.1; NP_665910.1; NP_665912.1).
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Chinese hamster
  • Immunogen

    Synthetic peptide corresponding to Human HMGA1 aa 12-23 (internal sequence) (Cysteine residue). NP_665906.1; NP_665908.1; NP_002122.1; NP_665909.1; NP_665912.1; NP_665910.1.
    Sequence:

    C-LASKQEKDGTEK


    Database link: P17096

  • Positive control

    • IHC-P: Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab223658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 4 - 6 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  • Involvement in disease

    Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Post-translational
    modifications

    Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
    HMG-Y is not methylated.
    Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • High mobility group (nonhistone chromosomal) protein isoforms I and Y antibody
    • High mobility group AT hook 1 antibody
    • High mobility group AT-hook protein 1 antibody
    • High mobility group protein 1 antibody
    • High mobility group protein A1 antibody
    • High mobility group protein HMG-I/HMG-Y antibody
    • High mobility group protein Ia antibody
    • High mobility group protein R antibody
    • HMG R antibody
    • HMG-I(Y) antibody
    • HMGA1 antibody
    • HMGA1_HUMAN antibody
    • HMGA1A antibody
    • HMGIY antibody
    • MGC12816 antibody
    • MGC4242 antibody
    • MGC4854 antibody
    • Nonhistone chromosomal high mobility group protein HMG I/HMG Y antibody
    see all

Images

  • Paraffin-embedded human small intestine stained for HMGA1 using ab223658 at 4 μg/ml in immunohistochemical analysis.

  • Paraffin-embedded human placenta tissue stained for HMGA1 using ab223658 at 4 μg/ml in immunohistochemical analysis.

References

ab223658 has not yet been referenced specifically in any publications.

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