Anti-HMGA1 antibody (ab230384)
Key features and details
- Rabbit polyclonal to HMGA1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HMGA1 antibody
See all HMGA1 primary antibodies -
Description
Rabbit polyclonal to HMGA1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
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Immunogen
Synthetic peptide within Human HMGA1 aa 1-53. The exact sequence is proprietary.
Database link: P17096 -
Positive control
- IHC-P: Human testis tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab230384 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. |
Target
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Function
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. -
Involvement in disease
Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1. -
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
Post-translational
modificationsConstitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 746594 Chimpanzee
- Entrez Gene: 618849 Cow
- Entrez Gene: 442946 Dog
- Entrez Gene: 3159 Human
- Entrez Gene: 15361 Mouse
- Entrez Gene: 100157848 Pig
- Entrez Gene: 117062 Rat
- Entrez Gene: 718536 Rhesus monkey
see all -
Alternative names
- High mobility group (nonhistone chromosomal) protein isoforms I and Y antibody
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA1 antibody (ab230384)
Formalin-fixed, paraffin-embedded human testis tissue stained for HMGA1 using ab230384 at 10 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (0)
ab230384 has not yet been referenced specifically in any publications.
