Recombinant Anti-HMGA1 antibody [EPR7839] - BSA and Azide free (ab226112)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7839] to HMGA1 - BSA and Azide free
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HMGA1 antibody [EPR7839] - BSA and Azide free
See all HMGA1 primary antibodies -
Description
Rabbit monoclonal [EPR7839] to HMGA1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MDA-MB-231 nuclear and membrane fraction lysates, MDA-MB-231 and HCT 116 whole cell lysates prepared in 1% SDS Hot lysis method, MDA-MB-231 and HCT 116 whole cell lysates prepared in RIPA lysis method, SK-OV-3 whole cell lysate. IHC-P: Human transitional cell carcinoma of bladder tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HepG2 cells.
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General notes
ab226112 is the carrier-free version of ab129153.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7839 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-HMGA1 antibody [EPR7839] (ab129153)
- Alexa Fluor® 488 Anti-HMGA1 antibody [EPR7839] (ab204667)
- PE Anti-HMGA1 antibody [EPR7839] (ab209761)
- APC Anti-HMGA1 antibody [EPR7839] - Nuclear Marker (ab310820)
- Alexa Fluor® 647 Anti-HMGA1 antibody [EPR7839] (ab311090)
- Alexa Fluor® 594 Anti-HMGA1 antibody [EPR7839] (ab311691)
- Alexa Fluor® 568 Anti-HMGA1 antibody [EPR7839] (ab312967)
- Alexa Fluor® 555 Anti-HMGA1 antibody [EPR7839] (ab313176)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
- Hep G2 nuclear extract lysate (ab14660)
- Mouse brain tissue lysate - total protein (ab30151)
- Mouse brain tissue lysate - total protein (ab4022)
- Mouse brain tissue lysate - total protein (0 days) (ab7188)
- Mouse brain tissue lysate - total protein (14 days) (ab7189)
- Mouse brain tissue lysate - total protein (7 days) (ab7190)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226112 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 17 kDa (predicted molecular weight: 12 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody. |
Target
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Function
HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. -
Involvement in disease
Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1. -
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
Post-translational
modificationsConstitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3159 Human
- Entrez Gene: 15361 Mouse
- Entrez Gene: 117062 Rat
- Omim: 600701 Human
- SwissProt: P17096 Human
- SwissProt: P17095 Mouse
- SwissProt: Q8K585 Rat
- Unigene: 518805 Human
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Alternative names
- High mobility group (nonhistone chromosomal) protein isoforms I and Y antibody
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
see all
Images
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Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling HMGA1 with purified ab129153 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
Control: primary antibody (1/500) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129153).
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Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/10000 dilution + SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129153).
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This IHC data was generated using the same anti-HMGA1 antibody clone, EPR7839, in a different buffer formulation (cat# ab129153).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue labelling HMGA1 with purified ab129153 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with Hematoxylin.
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All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/1000 dilution
Lane 1 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 2 : HCT 116 (Human colorectal carcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in RIPA lysis method
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate prepared in 1% SDS Hot lysis method
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
We recommend using 1% SDS Hot lysis prepare method to get desired Western Blot results.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129153).
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Intracellular Flow Cytometry analysis of HepG2 cells labelling HMGA1 with purified ab129153 at 1/60 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129153).
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All lanes : Anti-HMGA1 antibody [EPR7839] (ab129153) at 1/1000 dilution
Lane 1 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) nuclear fraction lysate
Lane 2 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) cytoplasm fraction lysate
Lane 3 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) membrane fraction lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 12 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM /TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab129153).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab226112 has been referenced in 1 publication.
- Hu J & Shen W microRNA-196a attenuates ischemic brain injury in rats by directly targeting high mobility group A1. Exp Ther Med 17:1579-1586 (2019). PubMed: 30783424