Overview

  • Product name

    Anti-HMGA1 antibody - N-terminal
    See all HMGA1 primary antibodies
  • Description

    Rabbit polyclonal to HMGA1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human HMGA1 (N terminal). The exact sequence is proprietary.
    Database link: P17096

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. ICC/IF: MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab226850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 12 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
  • Involvement in disease

    Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Post-translational
    modifications

    Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
    HMG-Y is not methylated.
    Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
  • Cellular localization

    Nucleus. Chromosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • High mobility group (nonhistone chromosomal) protein isoforms I and Y antibody
    • High mobility group AT hook 1 antibody
    • High mobility group AT-hook protein 1 antibody
    • High mobility group protein 1 antibody
    • High mobility group protein A1 antibody
    • High mobility group protein HMG-I/HMG-Y antibody
    • High mobility group protein Ia antibody
    • High mobility group protein R antibody
    • HMG R antibody
    • HMG-I(Y) antibody
    • HMGA1 antibody
    • HMGA1_HUMAN antibody
    • HMGA1A antibody
    • HMGIY antibody
    • MGC12816 antibody
    • MGC4242 antibody
    • MGC4854 antibody
    • Nonhistone chromosomal high mobility group protein HMG I/HMG Y antibody
    see all

Images

  • All lanes : Anti-HMGA1 antibody - N-terminal (ab226850) at 1/3000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lanes 2-4 : HMGA1 shRNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-HMGA1 antibody - N-terminal (ab226850) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS-PAGE gel.

  • 4% paraformaldehyde-fixed MCF7 (human breast adenocarcinoma cell line) cells labeling HMGA1 (green) using ab226850 at 1/500 dilution in ICC/IF. Nuclear Counterstain: Hoechst 33342.

References

ab226850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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