Key features and details
- Goat polyclonal to HMGA1a / HMGA1b
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HMGA1a / HMGA1b antibody
See all HMGA1a / HMGA1b primary antibodies
DescriptionGoat polyclonal to HMGA1a / HMGA1b
Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig
- WB: pancreas lysate. IHC-P: placenta or skin tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab109706 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 12 kDa.|
|IHC-P||Use a concentration of 3.75 µg/ml.|
FunctionHMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions.
Involvement in diseaseNote=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51L1.
Sequence similaritiesBelongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains.
modificationsConstitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoform HMG-Y can be phosphorylated by HIPK2.
HMG-Y is not methylated.
Methylation at Arg-58 is mutually exclusive with methylation at Arg-60.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- High mobility group AT hook 1 antibody
- High mobility group AT-hook protein 1 antibody
- High mobility group protein A1 antibody
Anti-HMGA1a / HMGA1b antibody (ab109706) at 0.5 µg/ml (incubation time: 1 hour) + Human pancreas lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 12 kDa
ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human placenta tissue.
ab109706 at 3.75 µg/ml staining formalin-fixed, paraffin-embedded Human skin tissue.
ab109706 has not yet been referenced specifically in any publications.