Overview

  • Product name
  • Description
    Rabbit polyclonal to HMGA2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HMGA2 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P52926

  • Positive control
    • SW579 cells. Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab52039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. PubMed: 23184966
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P 1/50.

Target

  • Function
    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • Involvement in disease
    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • Sequence similarities
    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Developmental stage
    Expressed predominantly during embryogenesis.
  • Post-translational
    modifications
    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Images

  • Human breast carcinoma stained with Anti-HMGA2 antibody, ab52039 at 1:50 for 30 min at RT
  • ICC/IF image of ab52039 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52039, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:
  • Xu L  et al. miR-541 suppresses proliferation and invasion of squamous cell lung carcinoma cell lines via directly targeting high-mobility group AT-hook 2. Cancer Med 7:2581-2591 (2018). Read more (PubMed: 29659195) »
  • Dom G  et al. Thyroid follicular adenomas and carcinomas: molecular profiling provides evidence for a continuous evolution. Oncotarget 9:10343-10359 (2018). Read more (PubMed: 29535811) »
See all 14 Publications for this product

Customer reviews and Q&As

Answer

Thank you for your inquiry.

We have a number of antibodies in our catalog that might be of interest to you. I can recommend to review the online datasheets for further information. Prices are provided at the top of the online datasheets.

HMGA1:

ab129153 HMGA1 antibody [EPR7839]
Rabbit monoclonal
Tested in:Flow Cyt, ICC/IF, IHC-P, WB
Reacts with: Human, Mouse, Rat
Price: euro370.00
https://www.abcam.com/index.html?datasheet=129153 (or use the following: https://www.abcam.com/index.html?datasheet=129153).

HMGA2:

ab52039 HMGA2 antibody
Rabbit polyclonal
Tested in: ICC/IF, IHC-P
Reacts with: Human
Price: euro370.00
https://www.abcam.com/index.html?datasheet=52039 (or use the following: https://www.abcam.com/index.html?datasheet=52039).

ab56390 HMGA2 antibody
Mouse monoclonal
Tested in: ICC, IHC-P
Reacts with: Human
Price: euro370.00
https://www.abcam.com/index.html?datasheet=56390 (or use the following: https://www.abcam.com/index.html?datasheet=56390).

ab52089 HMGA2 antibody, prediluted
Rabbit polyclonal
Tested in: IHC-P
Reacts with: Human
Price: euro370.00
https://www.abcam.com/index.html?datasheet=52089 (or use the following: https://www.abcam.com/index.html?datasheet=52089).

Shipping costs are also listed by the price on the datasheet. To Sweeden this is currently euro 22.00.

I can suggest you may like to try the search facility on our website (https://www.abcam.com). To do this, you can enter the name of the protein into the search box at the top of the home page. A list of products will be generated frmo all products types. if you then select the product type at the top of the page (eg primary antibody), this will provide a list of the correct product type which can then be further refined using the filters on the left hand side of the page. Using these filters will help you to identify products that have been tested in the applications and species you are interested in.

I hope this information is helpful to you. If you have specific questions about the products identified during the search, please do not hesitate to contact me.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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