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Anti-HMGA2 antibody (ab97276)

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  • SDS
Reviews (3)Q&A (1)References (27)

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Western blot - Anti-HMGA2 antibody (ab97276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
  • Immunoprecipitation - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)
  • Western blot - Anti-HMGA2 antibody (ab97276)

Key features and details

  • Rabbit polyclonal to HMGA2
  • Suitable for: IHC-P, IP, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-HMGA2 antibody
    See all HMGA2 primary antibodies
  • Description

    Rabbit polyclonal to HMGA2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human HMGA2 aa 1-93 (N terminal).
    Database link: P52926

  • Positive control

    • WB: MOLT-4, HepG2, HCT116, HeLa, A431, NIH/3T3, Raw264.7, C2C12, PC-12, Rat2 whole cell lysate. IP: HepG2 cells. ICC/IF: MCF7, HeLa cells. IHC-P: Rat brain, rat duodenum, human HuCCT1 xenograft, C2C12 xenograft.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Histones
    • HMGs
    • Neuroscience
    • Neurology process
    • Metabolism
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Differentiation
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Developmental Biology
    • Post embryonic development
    • Aging
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Raji whole cell lysate (ab30124)
    • MOLT-4 whole cell lysate (ab7912)
  • Recombinant Protein

    • Recombinant Human HMGA2 protein (ab156333)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab97276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/1000.
IP
1/100 - 1/500.
WB (2)
1/500 - 1/50000. Predicted molecular weight: 12 kDa.
ICC/IF
Use a concentration of 1 µg/ml.
Notes
IHC-P
1/100 - 1/1000.
IP
1/100 - 1/500.
WB
1/500 - 1/50000. Predicted molecular weight: 12 kDa.
ICC/IF
Use a concentration of 1 µg/ml.

Target

  • Function

    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
  • Involvement in disease

    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.
  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.
  • Developmental stage

    Expressed predominantly during embryogenesis.
  • Post-translational
    modifications

    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P52926 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8091 Human
    • Entrez Gene: 15364 Mouse
    • Entrez Gene: 84017 Rat
    • Omim: 600698 Human
    • SwissProt: P52926 Human
    • SwissProt: P52927 Mouse
    • Unigene: 505924 Human
    • Unigene: 157190 Mouse
    • Unigene: 29981 Rat
    see all
  • Alternative names

    • HMGA 2 antibody
    • BABL antibody
    • High mobility group (nonhistone chromosomal) protein isoform I C antibody
    • High mobility group (nonhistone chromosomal) protein isoform IC antibody
    • High Mobility Group AT hook 2 antibody
    • High Mobility Group AT hook protein 2 antibody
    • High mobility group AT-hook protein 2 antibody
    • High mobility group protein HMGI C antibody
    • High mobility group protein HMGI-C antibody
    • High mobility group protein HMGIC antibody
    • High mobility group protein I, isoform C antibody
    • HMGA2 antibody
    • HMGA2_HUMAN antibody
    • HMGI C antibody
    • HMGIC antibody
    • LIPO antibody
    • Non histone chromosomal architectural protein HMGI C antibody
    • Non histone chromosomal architectural protein HMGIC antibody
    • Pygmy antibody
    • STQTL9 antibody
    see all

Images

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

    Lane 1 : A431 whole cell extract
    Lane 2 : HeLa whole cell extract
    Lane 3 : HepG2 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)

    Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)

    HeLa cells stained for HMGA2 (green) using ab97276 at 1/500 dilution in ICC/IF. 

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution

    Lane 1 : NIH/3T3 whole cell extract
    Lane 2 : Raw264.7 whole cell extract
    Lane 3 : C2C12 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)

    Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution

    Lane 1 : PC-12 whole cell extract
    Lane 2 : Rat2 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa



    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)

    Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    Anti-HMGA2 antibody (ab97276) at 1/10000 dilution + HCT116 whole cell extract at 30 µg

    Predicted band size: 12 kDa



    15% SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)

    Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.

  • Immunoprecipitation - Anti-HMGA2 antibody (ab97276)
    Immunoprecipitation - Anti-HMGA2 antibody (ab97276)

    Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.

    Lane 1: Input

    Lane 2: Immunoprecipitation using control IgG

    Lane 3: Immunoprecipitation using 5 μg ab97276 

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg

    Predicted band size: 12 kDa



    15% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)
    Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)

    ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)
    All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution

    Lane 1 : HepG2
    Lane 2 : HCT116

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 12 kDa

  • Western blot - Anti-HMGA2 antibody (ab97276)
    Western blot - Anti-HMGA2 antibody (ab97276)This image is courtesy of an anonymous abreview.
    Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg

    Secondary
    Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution

    Predicted band size: 12 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?


    Exposure time: 10 minutes

    See Abreview

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (27)

Publishing research using ab97276? Please let us know so that we can cite the reference in this datasheet.

ab97276 has been referenced in 27 publications.

  • Cai X  et al. Circ_0000267 promotes gastric cancer progression via sponging MiR-503-5p and regulating HMGA2 expression. Mol Genet Genomic Med 8:e1093 (2020). PubMed: 31845519
  • Sati S  et al. 4D Genome Rewiring during Oncogene-Induced and Replicative Senescence. Mol Cell 78:522-538.e9 (2020). PubMed: 32220303
  • West RC  et al. BRCA1 regulates HMGA2 levels in the Swan71 trophoblast cell line. Mol Reprod Dev 86:1663-1670 (2019). PubMed: 31410930
  • Yang S  et al. HMGA2 regulates acute myeloid leukemia progression and sensitivity to daunorubicin via Wnt/ß-catenin signaling. Int J Mol Med 44:427-436 (2019). PubMed: 31173171
  • Wu H  et al. Long non-coding RNA PCAT6 targets miR-204 to modulate the chemoresistance of colorectal cancer cells to 5-fluorouracil-based treatment through HMGA2 signaling. Cancer Med 8:2484-2495 (2019). PubMed: 30938104
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HMGA2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Cell lysate - whole cell (Primary Fetal Fibroblast)
Gel Running Conditions
Reduced Denaturing (Mini-PROTEAN­ TGX™ 4-20%)
Loading amount
50 µg
Specification
Primary Fetal Fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Nov 21 2016

ChIP abreview for Anti-HMGA2 antibody

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Detection step
Semiquantitative PCR
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Negative control
DW No-antibody
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
input (whole lysate)
Read More

Abcam user community

Verified customer

Submitted Sep 19 2014

Question

WB images: one shows 12 kDa and other 18 kDa - why?

Read More

Abcam community

Verified customer

Asked on Jan 23 2014

Answer

The predicted size of HMGA2 is at 12 kDa, but it is also reported to migrate at higher size around 17-19 kDa probably due to PTM . (see publication: http://www.ncbi.nlm.nih.gov/pubmed/15026339)

The Uniprot database also shows HMGA2 has several acetylation sites and phosphorylation sites http://www.uniprot.org/uniprot/P52926

Our testing results of ab97276 also shows 19 kDa major band in some cells, and 12 kDa major band in others. The difference may indicate different post-tranlational modification conditions of HMGA2 among these cells.

Read More

Sybille Rex

Abcam Scientific Support

Answered on Jan 23 2014

Western blot abreview for Anti-HMGA2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLA)
Loading amount
25 µg
Specification
HeLA
Gel Running Conditions
Reduced Denaturing (17.5)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Feb 01 2012

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