Anti-HMGA2 antibody (ab97276)
Key features and details
- Rabbit polyclonal to HMGA2
- Suitable for: IHC-P, IP, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HMGA2 antibody
See all HMGA2 primary antibodies -
Description
Rabbit polyclonal to HMGA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human HMGA2 aa 1-93 (N terminal).
Database link: P52926 -
Positive control
- WB: MOLT-4, HepG2, HCT116, HeLa, A431, NIH/3T3, Raw264.7, C2C12, PC-12, Rat2 whole cell lysate. IP: HepG2 cells. ICC/IF: MCF7, HeLa cells. IHC-P: Rat brain, rat duodenum, human HuCCT1 xenograft, C2C12 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/100 - 1/1000.
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| IP |
1/100 - 1/500.
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| WB | (2) |
1/500 - 1/50000. Predicted molecular weight: 12 kDa.
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| ICC/IF |
Use a concentration of 1 µg/ml.
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| Notes |
|---|
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IHC-P
1/100 - 1/1000. |
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IP
1/100 - 1/500. |
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WB
1/500 - 1/50000. Predicted molecular weight: 12 kDa. |
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ICC/IF
Use a concentration of 1 µg/ml. |
Target
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Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. -
Involvement in disease
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. -
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
Developmental stage
Expressed predominantly during embryogenesis. -
Post-translational
modificationsRegulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8091 Human
- Entrez Gene: 15364 Mouse
- Entrez Gene: 84017 Rat
- Omim: 600698 Human
- SwissProt: P52926 Human
- SwissProt: P52927 Mouse
- Unigene: 505924 Human
- Unigene: 157190 Mouse
see all -
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
Images
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : A431 whole cell extract
Lane 2 : HeLa whole cell extract
Lane 3 : HepG2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded C2C12 xenograft tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)HeLa cells stained for HMGA2 (green) using ab97276 at 1/500 dilution in ICC/IF.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : NIH/3T3 whole cell extract
Lane 2 : Raw264.7 whole cell extract
Lane 3 : C2C12 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded human HuCCT1 xenograft tissue stained for HMGA2 using ab97276 at 1/250 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/1000 dilution
Lane 1 : PC-12 whole cell extract
Lane 2 : Rat2 whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat duodenum tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/10000 dilution + HCT116 whole cell extract at 30 µg
Predicted band size: 12 kDa15% SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody (ab97276)Paraffin embedded rat brain tissue stained for HMGA2 using ab97276 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-HMGA2 antibody (ab97276)Immunoprecipitation of HMGA2 protein from HepG2 nuclear extracts using 5 μg of HMGA2 antibody. Western blot analysis was performed using HMGA2 antibody at 1/500 dilution. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT-4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : HepG2
Lane 2 : HCT116
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa -
Western blot - Anti-HMGA2 antibody (ab97276)This image is courtesy of an anonymous abreview.Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (27)
ab97276 has been referenced in 27 publications.
- Cai X et al. Circ_0000267 promotes gastric cancer progression via sponging MiR-503-5p and regulating HMGA2 expression. Mol Genet Genomic Med 8:e1093 (2020). PubMed: 31845519
- Sati S et al. 4D Genome Rewiring during Oncogene-Induced and Replicative Senescence. Mol Cell 78:522-538.e9 (2020). PubMed: 32220303
- West RC et al. BRCA1 regulates HMGA2 levels in the Swan71 trophoblast cell line. Mol Reprod Dev 86:1663-1670 (2019). PubMed: 31410930
- Yang S et al. HMGA2 regulates acute myeloid leukemia progression and sensitivity to daunorubicin via Wnt/ß-catenin signaling. Int J Mol Med 44:427-436 (2019). PubMed: 31173171
- Wu H et al. Long non-coding RNA PCAT6 targets miR-204 to modulate the chemoresistance of colorectal cancer cells to 5-fluorouracil-based treatment through HMGA2 signaling. Cancer Med 8:2484-2495 (2019). PubMed: 30938104