- Datasheet
- Specific References (6)
- Protocols
Overview
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Product nameAnti-HMGA2 antibody
See all HMGA2 primary antibodies -
DescriptionRabbit polyclonal to HMGA2
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, IP, WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).
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Positive control
- MOLT4 whole cell lysate, MCF7 cells, HepG2, HCT116, HeLa
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.21% Tris, 0.75% Glycine -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| IP | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 12 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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FunctionFunctions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.
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Involvement in diseaseNote=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. -
Sequence similaritiesBelongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
Developmental stageExpressed predominantly during embryogenesis.
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Post-translational
modificationsRegulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 8091 Human
- Entrez Gene: 15364 Mouse
- Entrez Gene: 84017 Rat
- Omim: 600698 Human
- SwissProt: P52926 Human
- SwissProt: P52927 Mouse
- Unigene: 505924 Human
- Unigene: 157190 Mouse
see all -
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
Images
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Immunoprecipitation - Anti-HMGA2 antibody (ab97276)Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : HepG2
Lane 2 : HCT116
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa -
Western blot - Anti-HMGA2 antibody (ab97276)This image is courtesy of an anonymous abreview.Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa (why is the actual band size different from the predicted?)
Exposure time: 10 minutes
Protocols
References
This product has been referenced in:
- Wang L et al. Increased high mobility group A 2 expression promotes transition of cervical intraepithelial neoplasm into cervical cancer. Oncotarget 9:7891-7901 (2018). Read more (PubMed: 29487700) »
- Ye ZH & Gui DW miR-539 suppresses proliferation and induces apoptosis in renal cell carcinoma by targeting high mobility group A2. Mol Med Rep 17:5611-5618 (2018). Read more (PubMed: 29436648) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"