Anti-HMGA2 antibody (ab97276)
Overview
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Product name
Anti-HMGA2 antibody
See all HMGA2 primary antibodies -
Description
Rabbit polyclonal to HMGA2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IP, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 1 - 93 of Human HMGA2 (NP_003474).
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Positive control
- MOLT4 whole cell lysate, MCF7 cells, HepG2, HCT116, HeLa
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab97276 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/1000. | |
| IP | 1/100 - 1/500. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 12 kDa. | |
| ICC/IF | Use a concentration of 1 µg/ml. |
Target
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Function
Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2. -
Involvement in disease
Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma. -
Sequence similarities
Belongs to the HMGA family.
Contains 3 A.T hook DNA-binding domains. -
Developmental stage
Expressed predominantly during embryogenesis. -
Post-translational
modificationsRegulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 8091 Human
- Entrez Gene: 15364 Mouse
- Entrez Gene: 84017 Rat
- Omim: 600698 Human
- SwissProt: P52926 Human
- SwissProt: P52927 Mouse
- Unigene: 505924 Human
- Unigene: 157190 Mouse
see all -
Alternative names
- HMGA 2 antibody
- BABL antibody
- High mobility group (nonhistone chromosomal) protein isoform I C antibody
see all
Images
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Immunoprecipitation - Anti-HMGA2 antibody (ab97276)Lane 1: Input
Lane 2: Immunoprecipitation using control IgG
Lane 3: Immunoprecipitation using 5 μg ab97276
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Western blot - Anti-HMGA2 antibody (ab97276)Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + MOLT4 whole cell lysate at 30 µg
Predicted band size: 12 kDa
15% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody (ab97276)ICC/IF image of ab97276 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab97276, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Western blot - Anti-HMGA2 antibody (ab97276)All lanes : Anti-HMGA2 antibody (ab97276) at 1/10000 dilution
Lane 1 : HepG2
Lane 2 : HCT116
Lysates/proteins at 30 µg per lane.
Predicted band size: 12 kDa -
Western blot - Anti-HMGA2 antibody (ab97276)This image is courtesy of an anonymous abreview.Anti-HMGA2 antibody (ab97276) at 1/1000 dilution + HeLa whole cell lysate at 25 µg
Secondary
Goat anti rabbit secondary IRDye 800CW at 1/1000 dilution
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 minutes
Protocols
References
ab97276 has been referenced in 18 publications.
- Wu H et al. Long non-coding RNA PCAT6 targets miR-204 to modulate the chemoresistance of colorectal cancer cells to 5-fluorouracil-based treatment through HMGA2 signaling. Cancer Med 8:2484-2495 (2019). PubMed: 30938104
- Wang T et al. Let-7a-5p may participate in the pathogenesis of diabetic nephropathy through targeting HMGA2. Mol Med Rep 19:4229-4237 (2019). PubMed: 30896854
- Yang S et al. HMGA2 regulates acute myeloid leukemia progression and sensitivity to daunorubicin via Wnt/ß-catenin signaling. Int J Mol Med 44:427-436 (2019). PubMed: 31173171
- Komuro A et al. Identification of a novel fusion gene HMGA2-EGFR in glioblastoma. Int J Cancer 142:1627-1639 (2018). PubMed: 29193056
- Zha Y et al. Hepatitis B virus X protein promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma cell line HCCLM3 by targeting HMGA2. Oncol Lett 16:5709-5714 (2018). PubMed: 30356986
