RecombinantRabMAb

Recombinant Anti-HMGA2 antibody [EPR18114] (ab207301)

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Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
  • Anti-HMGA2 antibody [EPR18114] (ab207301)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18114] to HMGA2
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-HMGA2 antibody [EPR18114]
    See all HMGA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18114] to HMGA2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human gastric cancer and colon cancer tissues. ICC/IF: HeLa and HepG2 cells. WB: HEK-293, HepG2 and NCCIT cells lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab207301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 12 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF (1)
1/100.
Notes
WB
1/1000. Predicted molecular weight: 12 kDa.
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100.

Target

  • Function

    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.

  • Involvement in disease

    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.

  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.

  • Developmental stage

    Expressed predominantly during embryogenesis.

  • Post-translational
    modifications

    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • HMGA 2 antibody
      • BABL antibody
      • High mobility group (nonhistone chromosomal) protein isoform I C antibody
      • High mobility group (nonhistone chromosomal) protein isoform IC antibody
      • High Mobility Group AT hook 2 antibody
      • High Mobility Group AT hook protein 2 antibody
      • High mobility group AT-hook protein 2 antibody
      • High mobility group protein HMGI C antibody
      • High mobility group protein HMGI-C antibody
      • High mobility group protein HMGIC antibody
      • High mobility group protein I, isoform C antibody
      • HMGA2 antibody
      • HMGA2_HUMAN antibody
      • HMGI C antibody
      • HMGIC antibody
      • LIPO antibody
      • Non histone chromosomal architectural protein HMGI C antibody
      • Non histone chromosomal architectural protein HMGIC antibody
      • Pygmy antibody
      • STQTL9 antibody
      see all

    Images

    • Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Western blot - Anti-HMGA2 antibody [EPR18114] (ab207301)
      All lanes : Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

      Lane 1 : HEK-293( Human embryonic kidney epithelial cell) 20 µg
      Lane 2 : HepG2 ( Human hepatocellular carcinoma epithelial cell)
      Lane 3 : NCCIT ( Human pluripotent embryonic carcinoma epithelial cell)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 12 kDa
      Observed band size: 18 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

      Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon cancer is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] (ab207301)

      Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon tissue. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

      The negative controls are as follows:
      -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)
      Immunocytochemistry/ Immunofluorescence - Anti-HMGA2 antibody [EPR18114] (ab207301)This image is courtesy of an abreview by Kirk Mcmanus

      ab207301 staining HMGA2 in the HeLal cell line from Human Cervix  by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldahyde, permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/400 in PBS) for 1 hour at 22°C. ab150081 (1/200)was used as the secondary antibody.

      See Abreview

    • Anti-HMGA2 antibody [EPR18114] (ab207301)
      Anti-HMGA2 antibody [EPR18114] (ab207301)

    References (1)

    Publishing research using ab207301? Please let us know so that we can cite the reference in this datasheet.

    ab207301 has been referenced in 1 publication.

    • Han X  et al. Long noncoding RNA VPS9D1-AS1 augments the malignant phenotype of non-small cell lung cancer by sponging microRNA-532-3p and thereby enhancing HMGA2 expression. Aging (Albany NY) 12:370-386 (2020). PubMed: 31902794

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-HMGA2 antibody [EPR18114]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Read More

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Aug 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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