RecombinantRabMAb

Recombinant Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

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Western blot - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
  • Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR18114] to HMGA2 - BSA and Azide free
  • Suitable for: WB, IHC-P, ICC
  • Reacts with: Human

Overview

  • Product name

    Anti-HMGA2 antibody [EPR18114] - BSA and Azide free
    See all HMGA2 primary antibodies

  • Description

    Rabbit monoclonal [EPR18114] to HMGA2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant full length protein within Human HMGA2 aa 1 to the C-terminus. The exact sequence is proprietary.
    Database link: P52926

  • General notes

    Ab251468 is the carrier-free version of ab207301. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab251468 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab251468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 12 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

Target

  • Function

    Functions as a transcriptional regulator. Functions in cell cycle regulation through CCNA2.

  • Involvement in disease

    Note=A chromosomal aberration involving HMGA2 is associated with a subclass of benign mesenchymal tumors known as lipomas. Translocation t(3;12)(q27-q28;q13-q15) with LPP is shown in lipomas. HMGA2 is also fused with a number of other genes in lipomas.
    Note=A chromosomal aberration involving HMGA2 is associated with pulmonary chondroid hamartomas. Translocation t(3;12)(q27-q28;q14-q15) with LPP is detected in pulmonary chondroid hamartomas.
    Note=A chromosomal aberration involving HMGA2 is associated with parosteal lipomas. Translocation t(3;12)(q28;q14) with LPP is also shown in one parosteal lipoma.
    Note=A chromosomal aberration involving HMGA2 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with RAD51L1. Chromosomal rearrangements involving HMGA2 do not seem to be the principle pathobiological mechanism in uterine leiomyoma.

  • Sequence similarities

    Belongs to the HMGA family.
    Contains 3 A.T hook DNA-binding domains.

  • Developmental stage

    Expressed predominantly during embryogenesis.

  • Post-translational
    modifications

    Regulated by cell cycle-dependent phosphorylation which alters its DNA binding affinity.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • HMGA 2 antibody
      • BABL antibody
      • High mobility group (nonhistone chromosomal) protein isoform I C antibody
      • High mobility group (nonhistone chromosomal) protein isoform IC antibody
      • High Mobility Group AT hook 2 antibody
      • High Mobility Group AT hook protein 2 antibody
      • High mobility group AT-hook protein 2 antibody
      • High mobility group protein HMGI C antibody
      • High mobility group protein HMGI-C antibody
      • High mobility group protein HMGIC antibody
      • High mobility group protein I, isoform C antibody
      • HMGA2 antibody
      • HMGA2_HUMAN antibody
      • HMGI C antibody
      • HMGIC antibody
      • LIPO antibody
      • Non histone chromosomal architectural protein HMGI C antibody
      • Non histone chromosomal architectural protein HMGIC antibody
      • Pygmy antibody
      • STQTL9 antibody
      see all

    Images

    • Western blot - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Western blot - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      All lanes : Anti-HMGA2 antibody [EPR18114] (ab207301) at 1/1000 dilution

      Lane 1 : HEK-293( Human embryonic kidney epithelial cell) 20 µg
      Lane 2 : HepG2 ( Human hepatocellular carcinoma epithelial cell)
      Lane 3 : NCCIT ( Human pluripotent embryonic carcinoma epithelial cell)

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 12 kDa
      Observed band size: 18 kDa
      why is the actual band size different from the predicted?



      This data was developed using ab207301, the same antibody clone in a different buffer formulation.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

      This data was developed using ab207301, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human gastric cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Nucleus staining on Human colon cancer is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling HMGA2 with ab207301 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative staining on Human colon tissue. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
    • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:
      -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
    • Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Immunocytochemistry - Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

      This data was developed using ab207301, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling HMGA2 with ab207301 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HepG2 cell line. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).The negative controls are as follows:
      -ve control 1: ab207301 at 1/100 dilution followed by ab150120 (Alexa Fluor® 594 Goat anti-Mouse secondary) at 1/1000 dilution.
      -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor® 488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

    • Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)
      Anti-HMGA2 antibody [EPR18114] - BSA and Azide free (ab251468)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab251468? Please let us know so that we can cite the reference in this datasheet.

    ab251468 has not yet been referenced specifically in any publications.

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