Immunocytochemistry/ Immunofluorescence abreview for Anti-HMGB1 antibody - ChIP Grade

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Pulmonary microvascular)
Permeabilization
Yes - 0.5% Triton in PBS
Specification
Pulmonary microvascular
Blocking step
BSA/NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Methanol:Acetone

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking buffer:PBS 1:10
Dilution
1/250

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Nov 15 2016

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