Western blot abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (melanoma)
Gel Running Conditions
Reduced Denaturing (12% gel)
Loading amount
20 µg
Treatment
radiation
Specification
melanoma
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/5000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 28 kDa

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Submitted Mar 08 2017

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