Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (tumor tissue)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
Yes - 0.3% triton X-100
Specification
tumor tissue
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: goat-anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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Submitted Jun 28 2017

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