Western blot abreview for Anti-HMGB1 antibody - ChIP Grade

Good
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS-T
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: LI-COR IRDye 800CW (827-08365)
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 800CW

Detection

Detection method
Fluorescence
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 30 kDa

Dr. SAIFUDEEN ISMAEL

Verified customer

Submitted Aug 03 2017

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