Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: R.T°C
Fixative
Paraformaldehyde

Other product details

Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/1000

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 594 Donkey antirabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 594

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Submitted Nov 01 2017

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