Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
1 hour(s) and 11 minute(s) · Temperature: 25°C · Diluent: PBS plus 1xcasein
Dilution
1/5000

Secondary antibody

Name
Non-Abcam antibody was used: ImmPRESS™ HRP Universal Antibody,Anti-Mouse/Rabbit
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

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Submitted Jun 19 2018

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