Immunocytochemistry/ Immunofluorescence abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (murin hepatocellular carcinoma)
Permeabilization
Yes - PBST(0.5% v/v triton X-100, sigma)
Specification
murin hepatocellular carcinoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: antibody diluent (GBI Labs)
Dilution
1/500

Secondary antibody

Dilution
1/200
Name
Non-Abcam antibody was used: AF488 anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

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Submitted Apr 04 2019

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