Western blot abreview for Anti-HMGB1 antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (brain)
Loading amount
40 µg
Specification
brain
Treatment
0.25ug/h LPS for 3 weeks
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Diluent: Blocking Buffer

Secondary antibody

Name
Non-Abcam antibody was used: Immiune pure Goat anti rabbit IgG (H+L) peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin

Detection

Detection method
ECL
Exposure
30 second(s)

Additional data

Additional Notes
The secondary antibody was peroxidase conjugated
We use a model witch is a chronic infusion of LPS in the 4th ventricle using an osmotic minipump paced under skin in the back o the animal and connected to a cannula that goes into the forth ventricle. Our minipum release 2ng of LPS per hour.
The chronic treatment with neramexane and memantine is still with the osmotic minipump in the back of the animal without beeing connected to a cannula.

A: rats infused with asf for 1 week
B: rats infusd with LPS for 1 week
C: rats infused with acsf for 8 weeks
D: rats infused with LPS for 8 weeks
E: rats infused with LPS 4 weeks
F: rats infused with LP for 4 weeks, after 2 weeks of LPS infusion were treated with neramexane for the next 2 weeks
G: rats infused with LPS for 4 weeks, after 2 weeks of LPS infusion were treated with memantine for the next 2 weeks.

Dr. Francesca Cerbai

Verified customer

Submitted Jul 17 2007

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