Product nameAnti-HMGB2 antibody
See all HMGB2 primary antibodies
DescriptionRabbit polyclonal to HMGB2
Tested applicationsSuitable for: ChIP, CHIPseq, IHC-P, WB, IP, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Opossum
- PC12 whole cell lysate. HEK-293T cell lysate. PC12 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityImmunogen affinity purified
ChIP Related Products
Our Abpromise guarantee covers the use of ab67282 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 21890638Use 5µg|
|CHIPseq||Use at an assay dependent concentration. PubMed: 27226577|
|IHC-P||Use at an assay dependent concentration. PubMed: 27226577|
|WB||Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 24 kDa.|
|IP||Use at an assay dependent concentration. Use 5-10µg.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
FunctionDNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS).
Sequence similaritiesBelongs to the HMGB family.
Contains 2 HMG box DNA-binding domains.
Cellular localizationNucleus. Chromosome.
- Information by UniProt
- C80539 antibody
- High mobility group (nonhistone chromosomal) protein 2 antibody
- High mobility group box 2 antibody
All lanes : Anti-HMGB2 antibody (ab67282) at 2 mg/ml
Lane 1 : NIH3T3
Lane 2 : Rat1
Lane 3 : RINSH
Lane 4 : PC12
Lane 5 : K562
Lane 6 : HEK-293T
Lane 7 : HeLa nuclear
Lane 8 : HeLa
Predicted band size: 24 kDa
Nuclear localization of HMGB2 in PC12 cells.
A. Cells were stained with Rabbit Anti-HMGB2 (ab67282) at 1 µg/mL
B. DAPI staining
C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2
Cross-linking chIP was performed using HMGB2 antibody (ab67282) and 25million HUVECs. The cells were crosslinked with 15mM ethylene glycol bis(succinimidylsuccinate) (EGS) for 20 minutes at room temperature, followed by the addition of Complete Cell Fixative Solution for 40 minutes at 4°C. 4µg of HMGB2 antibody was added per 25µg of sheared chromatin. qPCR was performed using positive primers against the 5’ prime UTR OF ZNF335 and HIST2H2AC genes and negative primer against the olfactory region 8.
1: 5 µg of Rabbit Anti-HMGB2 (ab67282) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate
2: Negative control – without cell lysate
3: Negative control – without antibody
4: Positive control – Extract of HEK-293T direct labeling
This product has been referenced in:
- Mun K & Punga T Cellular Zinc Finger Protein 622 Hinders Human Adenovirus Lytic Growth and Limits Binding of the Viral pVII Protein to Virus DNA. J Virol 93:N/A (2019). Read more (PubMed: 30429337) »
- Kimura A et al. HMGB2 expression is associated with transition from a quiescent to an activated state of adult neural stem cells. Dev Dyn 247:229-238 (2018). Read more (PubMed: 28771884) »