Recombinant Anti-HMGB2 antibody [EPR6301] (ab124670)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6301] to HMGB2
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-HMGB2 antibody [EPR6301]
See all HMGB2 primary antibodies -
Description
Rabbit monoclonal [EPR6301] to HMGB2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HMGB2 (N terminal). The exact sequence is proprietary.
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Positive control
- WB: HEK-293T, HAP1, K562, HeLa and PC12 cell lysates. ICC/IF: PC-12 cells. IHC-P: Human breast tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.03 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6301 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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KO cell pellets
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124670 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/500.
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WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 24 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Notes |
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ICC/IF
1/500. |
WB
1/10000 - 1/50000. Predicted molecular weight: 24 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
Target
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Function
DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). -
Sequence similarities
Belongs to the HMGB family.
Contains 2 HMG box DNA-binding domains. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 3148 Human
- Entrez Gene: 97165 Mouse
- Entrez Gene: 29395 Rat
- Entrez Gene: 498072 Rat
- Omim: 163906 Human
- SwissProt: P26583 Human
- SwissProt: P30681 Mouse
- SwissProt: P52925 Rat
see all -
Alternative names
- C80539 antibody
- High mobility group (nonhistone chromosomal) protein 2 antibody
- High mobility group box 2 antibody
see all
Images
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All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/2000 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : HMGB2 knockout HEK-293T cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1- 2: Merged signal (red and green). Green - ab124670 observed at 24 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab124670 was shown to react with HMGB2 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266358 (knockout cell lysate ab257156) was used. Wild-type HEK-293T and HMGB2 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab124670 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 2000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGB2 antibody [EPR6301] (ab124670)
ab124670, at a 1/250 dilution, staining HMGB2 in paraffin embedded Human breast tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling HMGB2 with ab124670 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI. -
All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HMGB2 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : K562 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab124670 observed at 24 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab124670 was shown to specifically react with HMGB2 in wild-type HAP1 cells as signal was lost in HMGB2 knockout cells. Wild-type and HMGB2 knockout samples were subjected to SDS-PAGE. Ab124670 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-HMGB2 antibody [EPR6301] (ab124670) at 1/10000 dilution
Lane 1 : K562 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : PC12 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 24 kDa -
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab124670 has been referenced in 10 publications.
- Lüdtke TH et al. Combined genomic and proteomic approaches reveal DNA binding sites and interaction partners of TBX2 in the developing lung. Respir Res 22:85 (2021). PubMed: 33731112
- Han X et al. Identification of differentially expressed proteins and clinicopathological significance of HMGB2 in cervical cancer. Clin Proteomics 18:2 (2021). PubMed: 33407071
- Li Y et al. microRNA-130a-5p suppresses myocardial ischemia reperfusion injury by downregulating the HMGB2/NF-?B axis. BMC Cardiovasc Disord 21:121 (2021). PubMed: 33658008
- Pu J et al. Long Noncoding RNA PART1 Promotes Hepatocellular Carcinoma Progression via Targeting miR-590-3p/HMGB2 Axis. Onco Targets Ther 13:9203-9211 (2020). PubMed: 32982307
- Olins AL et al. Hyperosmotic stress: in situ chromatin phase separation. Nucleus 11:1-18 (2020). PubMed: 31924112
- Zhang W et al. 6-Gingerol Attenuates Ischemia-Reperfusion-Induced Cell Apoptosis in Human AC16 Cardiomyocytes through HMGB2-JNK1/2-NF-?B Pathway. Evid Based Complement Alternat Med 2019:8798653 (2019). PubMed: 30886640
- Zhang Q et al. iTRAQ-based proteomic analysis of endotoxin tolerance induced by lipopolysaccharide. Mol Med Rep 20:584-592 (2019). PubMed: 31115557
- Zhang P et al. High-mobility group box 2 promoted proliferation of cervical cancer cells by activating AKT signaling pathway. J Cell Biochem 120:17345-17353 (2019). PubMed: 31209930
- Davies JE et al. Cross-reactivity of anti-HMGB1 antibodies for HMGB2. J Immunol Methods 456:72-76 (2018). PubMed: 29453955
- He YH et al. Association of Serum HMGB2 Levels With In-Stent Restenosis: HMGB2 Promotes Neointimal Hyperplasia in Mice With Femoral Artery Injury and Proliferation and Migration of VSMCs. Arterioscler Thromb Vasc Biol 37:717-729 (2017). PubMed: 28183701