Recombinant
RabMAb

Recombinant Anti-HMGCL antibody [EPR15551] (ab197022)

Overview

  • Product name

    Anti-HMGCL antibody [EPR15551]
    See all HMGCL primary antibodies
  • Description

    Rabbit monoclonal [EPR15551] to HMGCL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HMGCL aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: P35914

  • Positive control

    • WB: 293, Human testis and A431 cell lysates. IHC: Human liver tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab197022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).

Target

  • Function

    Involved in the catabolism of branched amino acids such as leucine.
  • Tissue specificity

    Fibroblasts, liver and lymphoblasts.
  • Pathway

    Metabolic intermediate metabolism; (S)-3-hydroxy-3-methylglutaryl-CoA degradation; acetoacetate from (S)-3-hydroxy-3-methylglutaryl-CoA: step 1/1.
  • Involvement in disease

    Defects in HMGCL are the cause of 3-hydroxy-3-methylglutaryl-CoA lyase deficiency (HMGCLD) [MIM:246450]; also known as hydroxymethylglutaricaciduria or HL deficiency. An autosomal recessive disease affecting ketogenesis and L-leucine catabolism. The disease usually appears in the first year of life after a fasting period and its clinical acute symptoms include vomiting, seizures, metabolic acidosis, hypoketotic hypoglycemia and lethargy. These symptoms sometimes progress to coma, with fatal outcome in some cases.
  • Sequence similarities

    Belongs to the HMG-CoA lyase family.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 3 hydroxy 3 methylglutaryl CoA lyase antibody
    • 3 hydroxy 3 methylglutaryl Coenzyme A lyase antibody
    • 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase (hydroxymethylglutaricaciduria) antibody
    • 3 hydroxymethyl 3 methylglutaryl Coenzyme A lyase antibody
    • 3-hydroxy-3-methylglutarate-CoA lyase antibody
    • HL antibody
    • HMG CoA lyase antibody
    • HMG CoA Lyase Deficiency antibody
    • HMG-CoA lyase antibody
    • HMGCL antibody
    • HMGCL_HUMAN antibody
    • Hydroxymethylglutaricaciduria antibody
    • Hydroxymethylglutaryl CoA lyase antibody
    • Hydroxymethylglutaryl CoA lyase mitochondrial antibody
    • Hydroxymethylglutaryl-CoA lyase antibody
    • Mitochondrial 3 hydroxy 3 methylglutaryl CoA lyase antibody
    • mitochondrial antibody
    • MS725 antibody
    • OTTHUMP00000044830 antibody
    see all

Images

  • Anti-HMGCL antibody [EPR15551] (ab197022) at 1/1000 dilution + 293 (Human epithelial cells from embryonic kidney) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-HMGCL antibody [EPR15551] (ab197022) at 1/5000 dilution + Human testis lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-HMGCL antibody [EPR15551] (ab197022) at 1/10000 dilution + A431 (Human epidermoid carcinoma) cell lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling HMGCL with ab197022 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.  Counter stained with Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab197022 and secondary antibody only.
    Note: Cytoplasm staining on human liver tissue was observed.

References

ab197022 has not yet been referenced specifically in any publications.

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