Recombinant
RabMAb

Anti-HMGCR antibody [EPR1685(N)] (ab174830)

Overview

  • Product name
    Anti-HMGCR antibody [EPR1685(N)]
    See all HMGCR primary antibodies
  • Description
    Rabbit monoclonal [EPR1685(N)] to HMGCR
  • Host species
    Rabbit
  • Specificity
    The antibody immunogen shares 93% homology with the mouse protein and 83% homology with the rat protein. We experienced some cross-reactivity variability with mouse and rat samples.
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HMGCR aa 400-500. The exact sequence is proprietary.
    Database link: P04035
    (Peptide available as ab209351)

  • Positive control
    • WB: HepG2, Jurkat, HeLa and TT cell lysates, Mouse and rat brain tissue lysates. IP: HepG2 cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 97 kDa.

For unpurified use at 1/100 - 1/500.

IP 1/10 - 1/100.

 

  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
    • Pathway
      Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 3/3.
    • Sequence similarities
      Belongs to the HMG-CoA reductase family.
    • Cellular localization
      Endoplasmic reticulum membrane. Peroxisome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3 hydroxy 3 methylglutaryl CoA reductase antibody
      • 3 hydroxy 3 methylglutaryl Coenzyme A reductase antibody
      • 3 hydroxymethylglutaryl CoA reductase antibody
      • 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) antibody
      • 3-hydroxy-3-methylglutaryl-coenzyme A reductase antibody
      • 3H3M antibody
      • HMDH_HUMAN antibody
      • HMG CoA reductase antibody
      • HMG CoAR antibody
      • HMG-CoA reductase antibody
      • Hmgcr antibody
      • Hydroxymethylglutaryl CoA reductase antibody
      • LDLCQ3 antibody
      • MGC103269 antibody
      • Red antibody
      see all

    Images

    • All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : TT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 97 kDa
      Observed band size: 97 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/3000 dilution (purified)

      Lane 1 : Mouse brain tissue lysate
      Lane 2 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size: 97 kDa
      Observed band size: 97 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM/TBST.

    • All lanes : Anti-HMGCR antibody [EPR1685(N)] (ab174830) at 1/1000 dilution (unpurified)

      Lane 1 : HepG2 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : TT cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 97 kDa

    • ab174830 (purified) at 1/100 immunoprecipitating HMGCR in HepG2 cell lysate. For western blotting, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    References

    This product has been referenced in:
    • Liang J  et al. Inhibition of the mevalonate pathway enhances cancer cell oncolysis mediated by M1 virus. Nat Commun 9:1524 (2018). Read more (PubMed: 29670091) »
    • Feng C  et al. Lysophosphatidylcholine acyltransferase 3 deficiency impairs 3T3L1 cell adipogenesis through activating Wnt/ß-catenin pathway. Biochim Biophys Acta 1863:834-843 (2018). Read more (PubMed: 29673706) »

    See all 21 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (macrophages)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    40 µg
    Specification
    macrophages
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 19 2018

    Application
    Western blot
    Sample
    Chinese hamster Cell lysate - whole cell (CHO cells)
    Gel Running Conditions
    Reduced Denaturing (10%)
    Loading amount
    50 µg
    Specification
    CHO cells
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Nov 14 2017

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HEK293)
    Permeabilization
    Yes - 0.1% Triton X100
    Specification
    HEK293
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jul 31 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3%TritonX100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Apr 24 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up