Anti-HMGCS2 antibody (ab157225)
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- References
- Protocols
Overview
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Product nameAnti-HMGCS2 antibody
See all HMGCS2 primary antibodies -
DescriptionRabbit polyclonal to HMGCS2
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HMGCS2.
Read Abcam's proprietary immunogen policy -
Positive control
- This antibody gave a positive signal in Human Liver tissue lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab157225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
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FunctionThis enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
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Tissue specificityHigh expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney.
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PathwayMetabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
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Involvement in diseaseDefects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids.
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Sequence similaritiesBelongs to the HMG-CoA synthase family.
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Cellular localizationMitochondrion.
- Information by UniProt
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Database links
- Entrez Gene: 3158 Human
- Omim: 600234 Human
- SwissProt: P54868 Human
- Unigene: 59889 Human
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Alternative names
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
- 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
- 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
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Images
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Western blot - Anti-HMGCS2 antibody (ab157225)Anti-HMGCS2 antibody (ab157225) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab157225 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Protocols
Datasheets and documents
References
ab157225 has not yet been referenced specifically in any publications.
