Overview

  • Product name
  • Description
    Rabbit polyclonal to HMGCS2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Macaque monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HMGCS2.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in Human Liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab157225 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).

Target

  • Function
    This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
  • Tissue specificity
    High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney.
  • Pathway
    Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
  • Involvement in disease
    Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids.
  • Sequence similarities
    Belongs to the HMG-CoA synthase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
    • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
    • 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
    • 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
    • HMCS2_HUMAN antibody
    • HMG CoA synthase antibody
    • HMG-CoA synthase antibody
    • HMGCS 2 antibody
    • HMGCS2 antibody
    • Hydroxymethylglutaryl CoA synthase antibody
    • Hydroxymethylglutaryl CoA synthase mitochondrial antibody
    • Hydroxymethylglutaryl-CoA synthase antibody
    • mitochondrial antibody
    see all

Images

  • Anti-HMGCS2 antibody (ab157225) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa
    Additional bands at: 77 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 10 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab157225 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab157225 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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