Overview

  • Product name

    Anti-HMGCS2 antibody [EPR8642]
    See all HMGCS2 primary antibodies
  • Description

    Rabbit monoclonal [EPR8642] to HMGCS2
  • Host species

    Rabbit
  • Specificity

    Does not cross react with HMGCS1.
  • Tested applications

    Suitable for: IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human HMGCS2 aa 450 to the C-terminus.
    Database link: P54868

  • Positive control

    • WB: Human fetal liver, mouse kidney and rat kidney tissue lysates and Caco 2 cell lysate. IHC-P: Human, mouse and rat liver tissues and human colon tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 57 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function

      This enzyme condenses acetyl-CoA with acetoacetyl-CoA to form HMG-CoA, which is the substrate for HMG-CoA reductase.
    • Tissue specificity

      High expression in liver and colon. Low expression in testis, heart, skeletal muscle and kidney.
    • Pathway

      Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.
    • Involvement in disease

      Defects in HMGCS2 are the cause of HMG-CoA synthase deficiency (HMGCS deficiency) [MIM:605911]; also known as deficiency of mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2. Affected individuals present with severe hypoketotic hypoglycemia, mild hepatomegaly, or fatty liver, and a nondiagnostic pattern of urinary organic acids with increase of medium and short chain dicarboxylic acids.
    • Sequence similarities

      Belongs to the HMG-CoA synthase family.
    • Cellular localization

      Mitochondrion.
    • Information by UniProt
    • Database links

    • Alternative names

      • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 (mitochondrial) antibody
      • 3 hydroxy 3 methylglutaryl Coenzyme A synthase 2 antibody
      • 3 hydroxy 3 methylglutaryl coenzyme A synthase antibody
      • 3-hydroxy-3-methylglutaryl coenzyme A synthase antibody
      • HMCS2_HUMAN antibody
      • HMG CoA synthase antibody
      • HMG-CoA synthase antibody
      • HMGCS 2 antibody
      • HMGCS2 antibody
      • Hydroxymethylglutaryl CoA synthase antibody
      • Hydroxymethylglutaryl CoA synthase mitochondrial antibody
      • Hydroxymethylglutaryl-CoA synthase antibody
      • mitochondrial antibody
      see all

    Images

    • Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/10000 dilution (purified) + Human fetal liver tissue lysate at 10 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size: 57 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (purified) + Rat kidney tissue lysate at 20 µg

      Secondary
      HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size: 57 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/5000 dilution (purified)

      Lane 1 : Caco-2 whole cell lysate
      Lane 2 : Mouse kidney tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG (specific to the non-reduced form of IgG) at 1/1000 dilution

      Predicted band size: 57 kDa
      Observed band size: 50 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM /TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with purified ab137043 at a dilution of 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • All lanes : Anti-HMGCS2 antibody [EPR8642] (ab137043) at 1/1000 dilution (unpurified)

      Lane 1 : Humn fetal liver lysate
      Lane 2 : Caco 2 lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling HMGCS2 with unpurified ab137043 at a diltuion of 1/50.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling HMGCS2 with unpurified ab137043 at a dilution of 1/50.

    References

    This product has been referenced in:

    • Zhang Z  et al. Genome-wide effect of pulmonary airway epithelial cell-specific Bmal1 deletion. FASEB J 33:6226-6238 (2019). Read more (PubMed: 30794439) »
    • Dhingra A  et al. Microtubule-Associated Protein 1 Light Chain 3B, (LC3B) Is Necessary to Maintain Lipid-Mediated Homeostasis in the Retinal Pigment Epithelium. Front Cell Neurosci 12:351 (2018). Read more (PubMed: 30349463) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    Answer


    Unfortunately, we do not have a strong explanation for your observation however, I found the following summary.

    http://www.proteinatlas.org/ENSG00000134240

    According to this, HMGCS2 was not detectable in HepG2 whereas it was observed medium abundance of HMGCS2 in Caco-2. Therefore is would appear that this discrepancy is not due to the antibody itself but to the sample used.

    Read More

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