Key features and details
- Rabbit polyclonal to HMGE
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HMGE antibody
See all HMGE primary antibodies
DescriptionRabbit polyclonal to HMGE
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Cow, Orangutan
Recombinant full length protein corresponding to Human HMGE aa 1-217.
MAAQCVRLARRSLPALALSLRPSPRLLCTATKQKNSGQNLEEDMGQSEQK ADPPATEKTLLEEKVKLEEQLKETVEKYKRALADTENLRQRSQKLVEEAK LYGIQAFCKDLLEVADVLEKATQCVPKEEIKDDNPHLKNLYEGLVMTEVQ IQKVFTKHGLLKLNPVGAKFDPYEHEALFHTPVEGKEPGTVALVSKVGYK LHGRTLRPALVGVVKEA
Database link: Q9HAV7
- WB: A431 and HEK-293T whole cell lysates. IHC-P: Human colon cancer and adrenal gland tissues.
Previously labelled as GRPEL1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230391 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Predicted molecular weight: 24 kDa.|
FunctionEssential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins.
Sequence similaritiesBelongs to the GrpE family.
Cellular localizationMitochondrion matrix.
- Information by UniProt
- FLJ25609 antibody
- GREPEL 1 antibody
- GREPEL1 antibody
All lanes : Anti-HMGE antibody (ab230391) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 24 kDa
Paraffin-embedded human colon cancer tissue stained for HMGE using ab230391 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human adrenal gland tissue stained for HMGE using ab230391 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230391 has not yet been referenced specifically in any publications.