Overview

  • Product name

  • Description

    Rabbit polyclonal to HMGN2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HMGN2 aa 2-35. The exact sequence is proprietary.
    Database link: P05204

  • Positive control

    • SH SY5Y cell lysate; HeLa cells and Human brain tissue

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride

    PBS is without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199679 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 9 kDa.
ICC/IF 1/100.

Target

  • Function

    Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
  • Sequence similarities

    Belongs to the HMGN family.
  • Post-translational
    modifications

    Phosphorylation favors cytoplasmic localization.
  • Cellular localization

    Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
  • Information by UniProt
  • Database links

  • Alternative names

    • High mobility group (nonhistone chromosomal) protein 17 antibody
    • high mobility group nucleosomal binding domain 2 antibody
    • High mobility group nucleosome-binding domain-containing protein 2 antibody
    • High mobility group protein N2 antibody
    • HMG17 antibody
    • HMGN2 antibody
    • HMGN2_HUMAN antibody
    • MGC5629 antibody
    • Non histone chromosomal protein HMG 17 antibody
    • Non-histone chromosomal protein HMG-17 antibody
    • Nonhistone chromosomal protein HMG 17 antibody
    • nonhistone chromosomal protein hmg-17 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells labeling HMGN2 using ab199679 at 1/100 dilution.

  • Anti-HMGN2 antibody (ab199679) at 1/1000 dilution + SH SY5Y cell lysate at 35 µg

    Secondary
    Goat anti-rabbit IgG H&L(HRP) at 1/5000 dilution

    Predicted band size: 9 kDa

  • Immunohistochemical analysis of Human brain tissue labeling HMGN2 using ab199679 at 1/50 dilution (left) and negative control (right).

References

This product has been referenced in:

  • Li Q  et al. Increased expression of high-mobility group nucleosomal-binding domain 2 protein in various tumor cell lines. Oncol Lett 15:4517-4522 (2018). Read more (PubMed: 29541221) »
See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Chicken Cell (retina)
Permeabilization
No
Specification
retina
Fixative
Paraformaldehyde

Mr. Alex Campbell

Verified customer

Submitted Mar 26 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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