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Rabbit monoclonal [EPR7091] to HMGN2
ICC more details Unsuitable for:
Flow Cyt or IP
Synthetic peptide within Human HMGN2 aa 50-150. The exact sequence is proprietary.
HL60, K562, HeLa, SH-SY5Y and Jurkat cell lysates; Human colon tissue.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in an unique chromatin conformation.
Belongs to the HMGN family.
Phosphorylation favors cytoplasmic localization.
Nucleus. Cytoplasm. Cytoplasmic enrichment upon phosphorylation.
Information by UniProt
High mobility group (nonhistone chromosomal) protein 17 antibody
high mobility group nucleosomal binding domain 2 antibody
High mobility group nucleosome-binding domain-containing protein 2 antibody
Western blot - Anti-HMGN2 antibody [EPR7091] (ab124997)
All lanes : Anti-HMGN2 antibody [EPR7091] (ab124997) at 1/1000 dilution Lane 1 : HL60 cell lysate Lane 2 : K562 cell lysate Lane 3 : HeLa cell lysate Lane 4 : SH-SY5Y cell lysate Lane 5 : Jurkat cell lysate Lysates/proteins at 10 µg per lane. Secondary All lanes : Goat anti-Rabbit HRP at 1/2000 dilution Predicted band size: 9 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HMGN2 antibody [EPR7091] (ab124997)
ab124997 at 1/250 dilution staining HMGN2 in paraffin-embedded Human colon tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"