Anti-HMGN3 antibody (ab72233)
Key features and details
- Rabbit polyclonal to HMGN3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HMGN3 antibody -
Description
Rabbit polyclonal to HMGN3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat -
Immunogen
Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.
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Positive control
- Whole cell lysate from Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function. -
Tissue specificity
Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries. -
Sequence similarities
Belongs to the HMGN family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100069315 Horse
- Entrez Gene: 9324 Human
- Omim: 604502 Human
- SwissProt: Q15651 Human
- Unigene: 77558 Human
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Alternative names
- DKFZp686E20226 antibody
- FLJ42187 antibody
- High mobility group nucleosomal binding domain 3 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB.
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All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 16 kDa, 51 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation/ Western Blot of HMGN3.
Lane 1: ab72233 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72233 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab72233 has been referenced in 2 publications.
- Sorin S et al. HMGN3 represses transcription of epithelial regulators to promote migration of cholangiocarcinoma in a SNAI2-dependent manner. FASEB J 36:e22345 (2022). PubMed: 35635715
- Yoffe Y et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev 30:1991-2004 (2016). PubMed: 27664238