Key features and details
- Rabbit polyclonal to HMGN3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HMGN3 antibody
DescriptionRabbit polyclonal to HMGN3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.
- Whole cell lysate from Hela cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab72233 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa).|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionBinds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.
Tissue specificityExpressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries.
Sequence similaritiesBelongs to the HMGN family.
- Information by UniProt
- DKFZp686E20226 antibody
- FLJ42187 antibody
- High mobility group nucleosomal binding domain 3 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB.
All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml
Lane 1 : Whole cell lysate from Hela cells at 50 µg
Lane 2 : Whole cell lysate from Hela cells at 15 µg
Lane 3 : Whole cell lysate from Hela cells at 5 µg
Predicted band size: 11 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 15 kDa, 16 kDa, 51 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation/ Western Blot of HMGN3.
Lane 1: ab72233 at 3µg/mg whole cell lysate. Lane 2: Control IgG.
ab72233 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.
ab72233 has been referenced in 1 publication.
- Yoffe Y et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev 30:1991-2004 (2016). PubMed: 27664238