• Product name
    Anti-HMGN3 antibody
  • Description
    Rabbit polyclonal to HMGN3
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat
  • Immunogen

    Synthetic peptide from a region between residue 60 and 99 of human HMGN3, NP_004233.1.

  • Positive control
    • Whole cell lysate from Hela cells.



Our Abpromise guarantee covers the use of ab72233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 15, 16, 19, 51 kDa (predicted molecular weight: 11 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Binds to nucleosomes, regulating chromatin structure and consequently, chromatin-dependent processes such as transcription, DNA replication and DNA repair. Affects both insulin and glucagon levels and modulates the expression of pancreatic genes involved in insulin secretion. Regulates the expression of the glucose transporter SLC2A2 by binding specifically to its promoter region and recruiting PDX1 and additional transcription factors. Regulates the expression of SLC6A9, a glycine transporter which regulates the glycine concentration in synaptic junctions in the central nervous system, by binding to its transcription start site. May play a role in ocular development and astrocyte function.
  • Tissue specificity
    Expressed in kidney, lung, pancreas, testis, skeletal muscle, heart, thyroid gland, pituitary gland, prostate and uterus. Low expression in liver, spleen, placenta and ovaries.
  • Sequence similarities
    Belongs to the HMGN family.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686E20226 antibody
    • FLJ42187 antibody
    • High mobility group nucleosomal binding domain 3 antibody
    • High mobility group nucleosome-binding domain-containing protein 3 antibody
    • HMGN3 antibody
    • HMGN3_HUMAN antibody
    • OTTHUMP00000016772 antibody
    • OTTHUMP00000016773 antibody
    • PNAS 24 antibody
    • PNAS 25 antibody
    • PNAS24 antibody
    • PNAS25 antibody
    • Thyroid hormone receptor interacting protein 7 antibody
    • Thyroid receptor-interacting protein 7 antibody
    • TR-interacting protein 7 antibody
    • TRIP 7 antibody
    • TRIP-7 antibody
    • TRIP7 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling HMGN3 with ab72233 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-HMGN3 antibody (ab72233) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from Hela cells at 50 µg
    Lane 2 : Whole cell lysate from Hela cells at 15 µg
    Lane 3 : Whole cell lysate from Hela cells at 5 µg

    Predicted band size: 11 kDa
    Observed band size: 19 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 15 kDa, 16 kDa, 51 kDa. We are unsure as to the identity of these extra bands.

  • Immunoprecipitation/ Western Blot of HMGN3.
    Lane 1: ab72233 at 3µg/mg whole cell lysate.
    Lane 2: Control IgG.
    ab72233 at 1µg/ml for WB.
    Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
    Chemiluminescence with an exposure time of 3 seconds.


This product has been referenced in:
  • Yoffe Y  et al. Cap-independent translation by DAP5 controls cell fate decisions in human embryonic stem cells. Genes Dev 30:1991-2004 (2016). Read more (PubMed: 27664238) »

See 1 Publication for this product

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