Anti-HMGN5 antibody (ab186001)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HMGN5 antibody
See all HMGN5 primary antibodies -
Description
Rabbit polyclonal to HMGN5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Recombinant fragment within Human HMGN5 aa 232-282 (C terminal). The exact sequence is proprietary. NP_110390.1
Database link: P82970 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
This product was previously labelled as NSBP1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab186001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 32 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the HMGN family. -
Domain
Specifically targeted by its C-terminus to nucleosomes in euchromatin. -
Cellular localization
Nucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 79366 Human
- Omim: 300385 Human
- SwissProt: P82970 Human
- Unigene: 282204 Human
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Alternative names
- High mobility group nucleosome binding domain 5 antibody
- High mobility group nucleosome-binding domain-containing protein 5 antibody
- Hmgn5 antibody
see all
Images
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Western blot - Anti-HMGN5 antibody (ab186001)All lanes : Anti-HMGN5 antibody (ab186001) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-HMGN5 antibody (ab186001)Detection of Human HMGN5 by Western Blot of Immunoprecipitates.
Lane 1: ab186001 used for immunoprecipitation (IP) at 6 μg/mg of lysate from HeLa cells (1 mg for IP; 20% of IP loaded)
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
References
ab186001 has not yet been referenced specifically in any publications.
