Anti-HMGN5 antibody (ab186001)
Key features and details
- Rabbit polyclonal to HMGN5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HMGN5 antibody
See all HMGN5 primary antibodies -
Description
Rabbit polyclonal to HMGN5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
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Immunogen
Recombinant fragment within Human HMGN5 aa 232-282 (C terminal). The exact sequence is proprietary. NP_110390.1
Database link: P82970 -
Positive control
- HeLa and 293T whole cell lysates.
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General notes
This product was previously labelled as NSBP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab186001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 32 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Preferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Belongs to the HMGN family. -
Domain
Specifically targeted by its C-terminus to nucleosomes in euchromatin. -
Cellular localization
Nucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin. - Information by UniProt
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Database links
- Entrez Gene: 79366 Human
- Omim: 300385 Human
- SwissProt: P82970 Human
- Unigene: 282204 Human
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Alternative names
- High mobility group nucleosome binding domain 5 antibody
- High mobility group nucleosome-binding domain-containing protein 5 antibody
- Hmgn5 antibody
see all
Images
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Western blot - Anti-HMGN5 antibody (ab186001)All lanes : Anti-HMGN5 antibody (ab186001) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-HMGN5 antibody (ab186001)Detection of Human HMGN5 by Western Blot of Immunoprecipitates.
Lane 1: ab186001 used for immunoprecipitation (IP) at 6 μg/mg of lysate from HeLa cells (1 mg for IP; 20% of IP loaded)
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
References (1)
ab186001 has been referenced in 1 publication.
- Xu E et al. Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis. Biomed Res Int 2019:5630124 (2019). PubMed: 31930127
