Key features and details
- Rabbit polyclonal to HMGN5
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HMGN5 antibody
See all HMGN5 primary antibodies
DescriptionRabbit polyclonal to HMGN5
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Recombinant fragment within Human HMGN5 aa 232-282 (C terminal). The exact sequence is proprietary. NP_110390.1
Database link: P82970
- HeLa and 293T whole cell lysates.
This product was previously labelled as NSBP1
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab186001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 32 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPreferentially binds to euchromatin and modulates cellular transcription by counteracting linker histone-mediated chromatin compaction.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the HMGN family.
DomainSpecifically targeted by its C-terminus to nucleosomes in euchromatin.
Cellular localizationNucleus. Associates with nucleosomes in euchromatin and is largely excluded from constitutive heterochromatin.
- Information by UniProt
- High mobility group nucleosome binding domain 5 antibody
- High mobility group nucleosome-binding domain-containing protein 5 antibody
- Hmgn5 antibody
All lanes : Anti-HMGN5 antibody (ab186001) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 32 kDa
Exposure time: 30 seconds
Detection of Human HMGN5 by Western Blot of Immunoprecipitates.
Lane 1: ab186001 used for immunoprecipitation (IP) at 6 μg/mg of lysate from HeLa cells (1 mg for IP; 20% of IP loaded)
Lane 2: Control IgG.
Detection: Chemiluminescence with an exposure time of 10 seconds.
ab186001 has been referenced in 1 publication.
- Xu E et al. Hypoxia-Inducible Factor 1A Upregulates HMGN5 by Increasing the Expression of GATA1 and Plays a Role in Osteosarcoma Metastasis. Biomed Res Int 2019:5630124 (2019). PubMed: 31930127