Key features and details
- Rabbit polyclonal to HMT
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HMT antibody
See all HMT primary antibodies
DescriptionRabbit polyclonal to HMT
ab196611 detects endogenous level of total HMT protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein within Human HMT. The exact sequence is proprietary.
Database link: P50135
- HT29, HepG2 and HeLa cell lysates; mouse liver, kidney and brain lysates. Human thyroid cancer, breast cancer, kidney cancer and stomach cancer tissues. U2OS cells.
Previously labelled as HNMT.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 49% PBS
PBS without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/500 - 1/2000. Predicted molecular weight: 33 kDa.|
FunctionInactivates histamine by N-methylation. Plays an important role in degrading histamine and in regulating the airway response to histamine.
Sequence similaritiesBelongs to the methyltransferase superfamily. HNMT family.
- Information by UniProt
- EC 18.104.22.168 antibody
- Histamine N methyltransferase antibody
- Histamine N-methyltransferase antibody
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue labeling HMT using ab196611 at a 1/200 dilution.
All lanes : Anti-HMT antibody (ab196611) at 1/500 dilution
Lane 1 : HT29 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : mouse liver lysate
Lane 5 : mouse kidney lysate
Lane 6 : mouse brain lysate
Predicted band size: 33 kDa
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling HMT using ab196611 at a 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue labeling HMT using ab196611 at a 1/200 dilution.
Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labeling HMT using ab196611 at a 1/200 dilution.
Immunofluorescence analysis of U2OS cells, labeling HMT using ab196611 at a 1/50 dilution. Blue: DAPI for nuclear staining.
ab196611 has not yet been referenced specifically in any publications.