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Synthetic peptide corresponding to Human HMT (N terminal) conjugated to keyhole limpet haemocyanin.
Our Abpromise guarantee covers the use of ab63147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||1/50 - 1/100. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).|
|IHC-P||1/10 - 1/50.|
ICC/IF image of ab63147 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63147, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of HMT expression in paraffin embedded formalin fixed human hepatocarcinoma tissue using 1/10 ab63147, followed by peroxidase-conjugated secondary antibody step and DAB staining.
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