Key features and details
- Rabbit polyclonal to HN1L/L11
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HN1L/L11 antibody
See all HN1L/L11 primary antibodies
DescriptionRabbit polyclonal to HN1L/L11
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
This product was previously labelled as HN1L
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab200587 was affinity purified using an epitope specific to HN1L/L11 immobilized on solid support.
Our Abpromise guarantee covers the use of ab200587 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 20 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
Tissue specificityExpressed in liver, kidney, prostate, testis and uterus.
Sequence similaritiesBelongs to the HN1 family.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- C16orf34 antibody
- Chromosome 16 open reading frame 34 antibody
- CRAMP1 like antibody
All lanes : Anti-HN1L/L11 antibody (ab200587) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 20 kDa
Exposure time: 3 minutes
Detection of HN1L/L11 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab200587 at 6 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection (Lane 1). Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 30 seconds.
ab200587 has been referenced in 1 publication.
- Kenny PJ et al. The FMRP-MOV10 complex: a translational regulatory switch modulated by G-Quadruplexes. Nucleic Acids Res 48:862-878 (2020). PubMed: 31740951