Anti-hnRNP K antibody [3C2] (ab39975)
Key features and details
- Mouse monoclonal [3C2] to hnRNP K
- Suitable for: IP, ELISA, ChIP/Chip, WB, ICC/IF, Flow Cyt
- Reacts with: Mouse, Rat, Hamster, Cow, Human, Xenopus laevis
- Isotype: IgG2b
Overview
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Product name
Anti-hnRNP K antibody [3C2]
See all hnRNP K primary antibodies -
Description
Mouse monoclonal [3C2] to hnRNP K -
Host species
Mouse -
Tested applications
Suitable for: IP, ELISA, ChIP/Chip, WB, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Cow, Human, Xenopus laevis -
Immunogen
Full length native protein (purified) (Human)
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Positive control
- HeLa extracts. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3C2 -
Myeloma
Sp2/0 -
Isotype
IgG2b -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | (1) |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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ChIP/Chip |
Use at an assay dependent concentration. PubMed: 20673990
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WB | (1) |
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
ChIP/Chip
Use at an assay dependent concentration. PubMed: 20673990 |
WB
1/1000. Detects a band of approximately 65 kDa (predicted molecular weight: 51 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
One of the major pre-mRNA-binding proteins. Binds tenaciously to poly(C) sequences. Likely to play a role in the nuclear metabolism of hnRNAs, particularly for pre-mRNAs that contain cytidine-rich sequences. Can also bind poly(C) single-stranded DNA. -
Sequence similarities
Contains 3 KH domains. -
Post-translational
modificationsArg-296 and Arg-299 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Cytoplasm. Nucleus > nucleoplasm. In case of ASFV infection, there is a shift in the localization which becomes predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 528135 Cow
- Entrez Gene: 3190 Human
- Entrez Gene: 15387 Mouse
- Entrez Gene: 117282 Rat
- Omim: 600712 Human
- SwissProt: Q3T0D0 Cow
- SwissProt: P61978 Human
- SwissProt: P61979 Mouse
see all -
Alternative names
- CSBP antibody
- dC stretch binding protein antibody
- FLJ41122 antibody
see all
Images
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All lanes : Anti-hnRNP K antibody [3C2] (ab39975) at 1/1000 dilution
Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 2 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 51 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible post-translational modification) -
ICC/IF image of ab39975 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab39975 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells stained with ab39975 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab39975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (33)
ab39975 has been referenced in 33 publications.
- Tan Y et al. A novel oncogenic seRNA promotes nasopharyngeal carcinoma metastasis. Cell Death Dis 13:401 (2022). PubMed: 35461306
- Xu Y et al. Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa. Front Cell Dev Biol 9:652809 (2021). PubMed: 34336820
- Booy EP et al. The noncoding RNA BC200 associates with polysomes to positively regulate mRNA translation in tumor cells. J Biol Chem 296:100036 (2021). PubMed: 33410401
- Zhao S et al. The RNA binding protein hnRNPK protects against adriamycin-induced podocyte injury. Ann Transl Med 9:1303 (2021). PubMed: 34532440
- Leidal AM et al. The LC3-conjugation machinery specifies the loading of RNA-binding proteins into extracellular vesicles. Nat Cell Biol 22:187-199 (2020). PubMed: 31932738