Anti-hnRNP U/p120 antibody [3G6] (ab10297)
Key features and details
- Mouse monoclonal [3G6] to hnRNP U/p120
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-hnRNP U/p120 antibody [3G6]
See all hnRNP U/p120 primary antibodies -
Description
Mouse monoclonal [3G6] to hnRNP U/p120 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Does not react with: Chicken, Lizard -
Immunogen
Full length native protein (purified) corresponding to Human hnRNP U/p120. Crosslinked RNPs eluted from an oligo (dt) cellulose column.
Database link: Q00839 -
Positive control
- WB: HeLa cytoplasmic and nuclear fraction. HEK-293T whole cell lysate. ICC/IF: U-2 OS cells. IHC-P: Human cerebellum tissue. Flow Cytometry: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Protein A purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
3G6 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10297 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa).
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IHC-P | (2) |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 97 kDa (predicted molecular weight: 120 kDa). |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
Post-translational
modificationsExtensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
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Database links
- Entrez Gene: 3192 Human
- Omim: 602869 Human
- SwissProt: Q00839 Human
- Unigene: 106212 Human
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Alternative names
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
Images
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All lanes : Anti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/1000 dilution
Lane 1 : Cytoplasmic fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Lane 2 : Nuclear fraction of HeLa (human epithelial cell line from cervix adenocarcinoma) lysate
Predicted band size: 120 kDa
Observed band size: 120 kDa
This correlates with the information published in the reference Dreyfuss, G. et. al. -
Anti-hnRNP U/p120 antibody [3G6] (ab10297) at 1/5000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 100 µg
Secondary
HRP conjugated sheep anti-mouse IgG
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 120 kDa
Observed band size: 120 kDa
Additional bands at: 60 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 1 minute
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ab10297 (4 µg/ml)) staining hnRNP U/p120 in human cerebellum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining of Purkinje cells neurons.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with a protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Overlay histogram showing HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained with ab10297 (red line). The cells were fixed with 100% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab10297, 1 µg/1x106 cells) for 30 minutes at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 minutes)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (28)
ab10297 has been referenced in 28 publications.
- Kolpa HJ et al. SAF-A mutants disrupt chromatin structure through dominant negative effects on RNAs associated with chromatin. Mamm Genome 33:366-381 (2022). PubMed: 34859278
- Zhao L et al. A Long Non-coding RNA IVRPIE Promotes Host Antiviral Immune Responses Through Regulating Interferon ß1 and ISG Expression. Front Microbiol 11:260 (2020). PubMed: 32153544
- Zietzer A et al. The RNA-binding protein hnRNPU regulates the sorting of microRNA-30c-5p into large extracellular vesicles. J Extracell Vesicles 9:1786967 (2020). PubMed: 32944175
- Sharp JA et al. Cell division requires RNA eviction from condensing chromosomes. J Cell Biol 219:N/A (2020). PubMed: 33053167
- Sun WL et al. Long noncoding RNA OIP5-AS1 targets Wnt-7b to affect glioma progression via modulation of miR-410. Biosci Rep 39:N/A (2019). PubMed: 30498093