Key features and details
- Goat polyclonal to HNF-1B
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HNF-1B antibody
See all HNF-1B primary antibodies
DescriptionGoat polyclonal to HNF-1B
SpecificityThis antibody is expected to recognize both reported isoforms
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
- Human Liver lysate
Previously labelled as HNF1 beta.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab59118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
1 hour primary incubation is recommended for this product.
|ICC/IF||Use at an assay dependent concentration.|
FunctionTranscription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
Involvement in diseaseDefects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy.
Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11) [MIM:611955]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
Sequence similaritiesBelongs to the HNF1 homeobox family.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- FJHN antibody
- Hepatocyte nuclear factor 1 beta antibody
- Hepatocyte nuclear factor 1-beta antibody
Anti-HNF-1B antibody (ab59118) at 0.1 µg/ml + Human liver lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
The 100kDa band observed was successfully blocked by incubation with the immunizing peptide, however its identity is not known.
Definitive endoderm derived from culturing human embryonic stem cells (HUES4) with Inhibin beta a (ab50051) and Wnt3a (ab81484) was further differentiated to primitive gut tube as described in Kroon et al, Nat Biotechnol. (2008). KGF (ab9601) was added to the media to direct differentiation towards primitive gut tube. These images were taken 3 days after addition of KGF, where the primitive gut tube marker TCF2 (ab59118 in green) is present. Dapi staining is shown in blue. Magnification of top panels: 100x, bottom panels: 200x. Negative controls of fluorescently conjugated secondary antibodies only, showed no staining. For more information on this immunofluorescence, please see abreview.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab59118 has not yet been referenced specifically in any publications.