Overview

  • Product name

    Anti-HNF-1B antibody [EPR6334(2)]
    See all HNF-1B primary antibodies
  • Description

    Rabbit monoclonal [EPR6334(2)] to HNF-1B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human HNF-1B aa 500-600. The exact sequence is proprietary.

  • Positive control

    • Human fetal kidney and Caco 2 lysates.
  • General notes

    A trial size is available to purchase for this antibody.

     

    Previously labelled as HNF1 beta. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab128912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.
  • Target

    • Function

      Transcription factor, probably binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
    • Involvement in disease

      Defects in HNF1B are the cause of renal cysts and diabetes syndrome (RCAD) [MIM:137920]; also called maturity-onset diabetes of the young type 5 (MODY5) or familial hypoplastic glomerulocystic kidney disease (GCKD). RCAD is an autosomal dominant disorder comprising non-diabetic renal disease resulting from abnormal renal development, and diabetes, which in some cases occurs earlier than age 25 years and is thus consistent with a diagnosis of maturity-onset diabetes of the young (MODY5). The renal disease is highly variable and includes renal cysts, glomerular tufts, aberrant nephrogenesis, primitive tubules, irregular collecting systems, oligomeganephronia, enlarged renal pelves, abnormal calyces, small kidney, single kidney, horseshoe kidney, and hyperuricemic nephropathy.
      Defects in HNF1B may be rare genetic risk factor contributing to the development of non-insulin-dependent diabetes mellitus (NIDDM) [MIM:125853]. NIDDM is characterized by an autosomal dominant mode of inheritance, onset during adulthood and insulin resistance.
      Defects in HNF1B may be a cause of susceptibility to prostate cancer hereditary type 11 (HPC11) [MIM:611955]. It is a condition associated with familial predisposition to cancer of the prostate. Most prostate cancers are adenocarcinomas that develop in the acini of the prostatic ducts. Other rare histopathologic types of prostate cancer that occur in approximately 5% of patients include small cell carcinoma, mucinous carcinoma, prostatic ductal carcinoma, transitional cell carcinoma, squamous cell carcinoma, basal cell carcinoma, adenoid cystic carcinoma (basaloid), signet-ring cell carcinoma and neuroendocrine carcinoma.
    • Sequence similarities

      Belongs to the HNF1 homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • FJHN antibody
      • Hepatocyte nuclear factor 1 beta antibody
      • Hepatocyte nuclear factor 1-beta antibody
      • HNF 1B antibody
      • HNF 2 antibody
      • HNF-1-beta antibody
      • HNF-1B antibody
      • HNF1 beta antibody
      • HNF1 homeobox B antibody
      • HNF1B antibody
      • HNF1B_HUMAN antibody
      • HNF1beta antibody
      • HNF2 antibody
      • Homeoprotein LF B3 antibody
      • Homeoprotein LFB3 antibody
      • HPC11 antibody
      • LF B3 antibody
      • LFB3 antibody
      • MODY 5 antibody
      • MODY5 antibody
      • TCF 2 antibody
      • TCF 2 protein antibody
      • TCF-2 antibody
      • TCF2 antibody
      • TCF2 protein antibody
      • Transcription factor 2 antibody
      • Transcription factor 2 hepatic antibody
      • Variant hepatic nuclear factor 1 antibody
      • Variant hepatic nuclear factor antibody
      • VHNF 1 antibody
      • vHNF1 antibody
      see all

    Images

    • All lanes : Anti-HNF-1B antibody [EPR6334(2)] (ab128912) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Caco 2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 61 kDa

    References

    This product has been referenced in:

    • Liu Q  et al. Disruption of mesoderm formation during cardiac differentiation due to developmental exposure to 13-cis-retinoic acid. Sci Rep 8:12960 (2018). Read more (PubMed: 30154523) »
    See 1 Publication for this product

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