Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Loading amount
20 µg
Specification
liver cancer
Gel Running Conditions
Reduced Denaturing (10%gel)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: primary antibody dilution solution
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: DyLight® 488
Dilution
1/5000
Detection
Detection method
Fluorescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50-60 kDa Non-specific: 45 kDa
Abcam user community
Verified customer
Submitted Oct 12 2012