Immunoprecipitation abreview for Anti-HNF4 antibody [K9218] - ChIP Grade

Average
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - nuclear (HK2)
Total protein in input
10 µg
Specification
HK2
Treatment
10ng/ml TGF-beta 1
Immuno-precipitation step
Other - Dynabeads® Protein A

Other product details

Concentration
1.5 µg/mg lysate
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Western Blot antibody

Name
Non-Abcam antibody was used: HNF-1alpha (H-140), Polyclonal Antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/500

Additional data

Additional Notes
The literature reports an association between HNF1 and 4, but we were unable to detect this by IP. This was nothing to do with the HNF4 antibody from abcam, as it worked well as a primary antibody in WB.

Dr. Robert Jenkins

Verified customer

Submitted Apr 12 2013

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