Western blot abreview for Anti-HNF-4-alpha antibody [K9218] - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (human liver carcinoma cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
100 µg
Specification
human liver carcinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk-TBS/T
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: ECL Mouse IgG, HRP-linked whole Ab (from sheep)
Host species: Sheep
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 53 kDa

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Submitted Aug 04 2015

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