Immunoprecipitation abreview for Anti-HNF-4-alpha antibody [K9218] - ChIP Grade

Excellent
Abreviews
Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (liver hepatocellular carcinoma cell line)
Total protein in input
1000 µg
Immuno-precipitation step
Protein G
Specification
liver hepatocellular carcinoma cell line

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Diluent: non-denaturing lysis buffer
Concentration
5 µg/mg lysate

Western Blot antibody

Dilution
1/1000

Additional data

Additional Notes
First lane, 100ug of total lysate input, lane on right side of gel is the IP (eluted from beads in 1X LDS buffer (life technologies) 95C, 3 min containing reducing reagent.

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Submitted Aug 04 2015

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