Immunocytochemistry/ Immunofluorescence abreview for Anti-HNF-4-alpha antibody [K9218] - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human liver organoid (paraffin-embedded))
Permeabilization
Yes - 1% normal goat serum PBST (0.4% Triton X)
Specification
Human liver organoid (paraffin-embedded)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1% normal goat serum PBST (o.4% TritonX)
Concentration
1 µg/ml

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluor 488 goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
The green colored positive staining was well shown in nuclei of the organoids. The 3 dimentional organoids fixed with formaldehyde were scrapped and embedded in Histogel. Then, the Histogel block having the organoids was re-embedded into paraffin. Citrate buffer (pH 6) was used to Ag retrieval step.

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Submitted Mar 01 2019

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