Overview

  • Product name
  • Description
    Rabbit Polyclonal to HNF1 alpha
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HNF1 aa 550-600. The exact sequence is proprietary.
    Sequence:

    SSESGLHTPAS QATTLHVPSQ DPAGIQHLQP AHRLSASPTV SSSSLVLYQS


    Database link: P20823

  • Positive control
    • HepG2 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab174653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 67 kDa.

Target

  • Function
    Transcriptional activator that regulates the tissue specific expression of multiple genes, especially in pancreatic islet cells and in liver. Required for the expression of several liver specific genes. Binds to the inverted palindrome 5'-GTTAATNATTAAC-3'.
  • Tissue specificity
    Liver.
  • Involvement in disease
    Defects in HNF1A are a cause of hepatic adenomas familial (HEPAF) [MIM:142330]. Hepatic adenomas are rare benign liver tumors of presumable epithelial origin that develop in an otherwise normal liver. Hepatic adenomas may be single or multiple. They consist of sheets of well-differentiated hepatocytes that contain fat and glycogen and can produce bile. Bile ducts or portal areas are absent. Kupffer cells, if present, are reduced in number and are non-functional. Conditions associated with adenomas are insulin-dependent diabetes mellitus and glycogen storage diseases (types 1 and 3). Note=Bi-allelic inactivation of HNF1A, whether sporadic or associated with MODY3, may be an early step in the developmant of some hepatocellular carcinomas.
    Defects in HNF1A are the cause of maturity-onset diabetes of the young type 3 (MODY3) [MIM:600496]; also symbolized MODY-3. MODY is a form of diabetes that is characterized by an autosomal dominant mode of inheritance, onset in childhood or early adulthood (usually before 25 years of age), a primary defect in insulin secretion and frequent insulin-independence at the beginning of the disease.
    Defects in HNF1A are the cause of susceptibility to diabetes mellitus insulin-dependent type 20 (IDDM20) [MIM:612520]. IDDM20 is a multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These features can result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
  • Sequence similarities
    Belongs to the HNF1 homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Albumin proximal factor antibody
    • Hepatic nuclear factor 1 alpha antibody
    • Hepatic nuclear factor 1 antibody
    • Hepatic transcription factor 1 alpha antibody
    • Hepatic transcription factor 1 antibody
    • Hepatocyte nuclear factor 1-alpha antibody
    • HNF 1 antibody
    • HNF 1A antibody
    • HNF-1-alpha antibody
    • HNF-1A antibody
    • hnf1a antibody
    • HNF1A_HUMAN antibody
    • Interferon production regulator factor antibody
    • LF B1 antibody
    • LF B1 hepatic nuclear factor antibody
    • LFB 1 antibody
    • LFB1 antibody
    • LFB1 hepatic nuclear factor antibody
    • Liver specific transcription factor LF B1 antibody
    • Liver specific transcription factor LFB1 antibody
    • Liver-specific transcription factor LF-B1 antibody
    • Maturity onset diabetes of the young 3 antibody
    • MODY 3 antibody
    • MODY3 antibody
    • TCF 1 antibody
    • TCF-1 antibody
    • TCF1 antibody
    • Transcription factor 1 antibody
    • Transcription factor 1 hepatic antibody
    see all

Images

  • Detection of HNF1 by Western Blot of Immunprecipitate.
    ab174653 at 1µg/ml labeling HNF1 in HepG2 whole cell lysate immunoprecipitated using ab174653 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

  • Anti-HNF1 alpha antibody (ab174653) at 0.100000001490116 µg/ml + HepG2 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 67 kDa


    Exposure time: 30 seconds

References

ab174653 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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